Dielectrophoresis to Analyze Mouse Hepatitis Coronavirus Particles
Description
Detection technologies and physical methods used for separation of complex molecules can be effective tools in research when applied to bioparticles including, but not limited to,
bacteria, viruses, and proteins. Dielectrophoresis (DEP) is a technique that has been used in
microfluidics for separation and concentration of bioparticles, with the benefits of not requiring
custom primers, utilizing small sample sizes, and relatively quick separation times for rapid
identification of pathogens such as viruses. As demonstrated in this study, a DEP device using
polydimethylsiloxane (PDMS) as an insulator was used for the identification and separation of a
mouse hepatitis coronavirus (MHV), a model coronavirus that only infects mice. Results indicate
that, using 10 microliters of MHV test sample diluted in buffer, the virus can be identified and
separated within 30 seconds using DC voltage of 800 V.
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2023
Agent
- Author (aut): mcfadden, matthew
- Thesis advisor (ths): Hogue, Brenda G
- Thesis advisor (ths): Hayes, Mark
- Committee member: Christen, Jennifer B
- Committee member: Wang, Shaopeng
- Publisher (pbl): Arizona State University