Heavy metals and persistent organic pollutants contribute to human health risks worldwide. Among the most common routes of exposure to pollutants for humans are through the consumption of contaminated water and food, with fish being among the greatest vectors for ingestion of heavy metals in humans, particularly mercury.This dissertation consists of three chapters with a central theme of investigating heavy metal and persistent organic pollutant concentrations in fish and corned beef, which are two commonly consumed food items in American Samoa. A literature review illustrated that historically the primary pollutants of concern in fish muscle tissue from American Samoa have been mercury, arsenic, and polycyclic aromatic hydrocarbon mixtures. To better understand the changes in heavy metals and persistent organic pollutants in fish, this study reports an updated data set, comparing concentrations in pollutants as they have changed over time. To further investigate pollutants in fish tissue, 77 locally caught and commonly consumed fish were analyzed for heavy metals and persistent organic pollutants, and baseline human health risk assessments were calculated for contaminants that had available oral reference doses. While in American Samoa collecting fish for contaminant analyses, it was realized that canned corned beef appeared to be more commonly consumed than fresh fish. An IRB approved consumption survey revealed that 89% of American Samoan adults regularly consume fish, which is the same percentage of people that reported eating canned corned beef, indicating a dramatic increase in this food item to their diet since its introduction in the 20th century. Results of this study indicate that fish muscle tissue generally has higher heavy metal concentrations than canned corned beef, and that mercury continues to be a main contaminant of concern when consuming fresh and canned fish in American Samoa. While none of the heavy metal concentrations in corned beef exceeded calculated action levels, these foods might contribute to negative health outcomes in other ways. One of the main findings of this study is that either the presence or the ability to detect persistent organic pollutant concentrations are increasing in fish tissue and should be periodically monitored to adequately reflect current conditions.

Marine plastic pollution (MPP) has emerged as one of the most pressing global environmental challenges of the anthropocene. There has been an upsurge in investment to mitigate MPP; however, interventions can be costly, inequitable, and ineffective in achieving their objectives. In my dissertation, I aim to research key considerations for creating cost-effective, equitable mitigation strategies for MPP and its impacts to marine biodiversity and coastal communities. In chapter one, I introduce the challenges plastic pollution poses. In chapter two, I use seascape ecology theory to present the concept of the plastic-scape and describe how seascape ecology principles, methods, and approaches to transdisciplinary science can inform research to mitigate MPP. In chapter three, I present a framework to help decision makers estimate the total cost of MPP interventions and partial costs accrued by stakeholder groups. I then apply this framework to two quantitative case studies and four comparative case studies to exemplify its use and highlight the ways spatial scale, temporal scale, and socio-economic conditions influence the intervention cost and cost distribution. In chapter four, I employ a trait-based approach to produce a framework for developing indices of species vulnerability to macroplastic pollution. Finally, in chapter five, I implement the framework developed in the previous chapter and present a multi-taxonomic, macroplastic vulnerability index for three marine taxa—mammals, birds, and turtles—to identify the marine species most vulnerable to macroplastic pollution in Hawai‘i. Overall, my dissertation shows how policy-driven, systemic research of MPP and its interventions can improve efforts to address MPP and its socio-economic and ecological consequences.

Advanced technology has increased access to Antarctica; consequently, there has been an increase in research and tourism. The production of the new technology and the increased number of individuals visiting can increase the presence of persistent organic pollutants and microplastic within Antarctic soil. Studies have focused primarily on identifying these pollutants in high human impact areas with perhaps an assumption that low human impact areas would have lower concentrations of pollutants. The object of this paper, therefore, was to test the hypothesis that higher concentrations of persistent organic pollutants and microplastic are found in soils collected near research stations and tourist areas, as opposed to sites that are further from stations and have less direct human impact. Soil samples were collected along a 1,500 km transect of the Scotia Arch and Antarctic Peninsula from three high human impact sites and three low human impact sites to compare the concentration of contaminates identified within the soil. The presence and quantities of microplastic were identified using Nile Red and fluorescence microscopy, while gas chromatography-mass spectrometry was used to detect polychlorinated biphenyls, pesticides, polycyclic aromatic hydrocarbons, n-alkane, and phthalates. Although varying contaminate concentration levels were found at all six sights, counter to the hypothesis, there were no clear patterns of increasing pollutants with increasing human activities. These findings could imply that global sources of pollutants can increase local pollutants indicating the best way to solve any pollution problem is through a global lens.

The intramandibular joint (IMJ) in Helostoma temminckii, also known as kissing gourami, is located in the lower jaw at the articulation of the dentary and angular-articular bones. These fish are known for a ‘kissing’ behavior, facilitated by the IMJ, which allows the jaws to protrude and rotate making the mouth appear to look like pursed lips. The IMJ has independently evolved in the teleost lineage multiple times, and the mechanics are slightly different in each lineage. In kissing gourami, there are no muscular connections to the region of the lower jaw anterior to the IMJ, thus the IMJ is something of an enigma in terms of how it is mobilized during feeding, and moreover returned to a resting state after feeding. High-speed video was used to examine lateral expansion particularly in the regions of the intramandibular joint (IMJ) and the quadratomandibular (QMJ) joint. Individuals were filmed from a lateral and ventral view simultaneously during food capture. From the data, it appears that the expansion at the IMJ contributes to lateral expansion of the head during food capture. Lateral expansion at the location of the IMJ appears to occur in tandem with rotation about that joint; however, lateral expansion in this species is also rather slight due to their extremely rigid heads.

Plastic pollution poses a threat to the health and well-being of marine mammals across the globe. This paper takes a previously developed trait-based risk assessment framework and applies it to all 118 species of marine mammals worldwide, to help create a relative ranking of vulnerability of species to plastic ingestion and entanglement. After extensive data collection on 13 traits related to each species’ relative likelihood of exposure to plastics, species sensitivity to plastic ingestion and entanglement, and overall population resiliency, the initial trait framework was adapted and scored to calculate the relative vulnerability of marine mammals to marine microplastic pollution. Results indicate that the Hawaiian Monk Seal has one of the highest relative vulnerabilities to macroplastic pollution among all marine mammals. Furthermore, this exercise highlighted several areas where future research is needed, including expanding the framework to microplastics, applying the framework to coastal human populations, and further investigation of unknown life history traits of various marine mammals.

There is an estimated five trillion pieces of plastic in the global ocean, with 4.8 to 12.7 million metric tons entering the ocean annually. Much of the plastic in the ocean is in the form of microplastics, or plastic particles <5mm in size. Microplastics enter the marine environment as primary or secondary microplastics; primary microplastics are pre-manufactured micro-sized particles, such as microbeads used in cosmetics, while secondary microplastics form from the degradation of larger plastic objects, such water bottles. Once in the ocean, plastics are readily colonized by a consortium of prokaryotic and eukaryotic organisms, which form dense biofilms on the plastic; this biofilm is termed the “plastisphere”. Despite growing concerns about the ecological impact of microplastics and their respective plastispheres on the marine environment, there is little consensus about the factors that shape the plastisphere on environmentally relevant secondary microplastics. The goal of my dissertation is to comprehensively analyze the role of plastic polymer type, incubation time, and geographic location on shaping plastisphere communities attached to secondary microplastics. I investigated the plastisphere of six chemically distinct plastic polymer types obtained from common household consumer products that were incubated in the coastal Caribbean (Bocas del Toro, Panama) and coastal Pacific (San Diego, CA) oceans. Genotyping using 16S and 18S rRNA gene amplification and next-generation Illumina sequencing was employed to identify bacterial and eukaryotic communities on the polymer surfaces. Statistical analyses show that there were no polymer-specific assemblages for prokaryotes or eukaryotes, but rather a microbial core community that was shared among plastic types. I also found that rare hydrocarbon degrading bacteria may be specific to certain chemical properties of the microplastics. Statistical comparisons of the communities across both sites showed that prokaryotic plastispheres were shaped primarily by incubation time and geographic location. Finally, I assessed the impact of biofilms on microplastic degradation and deposition and conclude that biofilms enhance microplastic sinking of negatively buoyant particles and reduce microplastic degradation. The results of my dissertation increases understanding of the factors that shape the plastisphere and how these communities ultimately determine the fate of microplastics in the marine environment.

Marine ecosystems are currently being impacted by various threats; however, quantification of the impacts of known threats and the population status of species are often conducted at different scales, depending upon stakeholder needs. Global-scale species assessments can mask the impact of local or regional threats within the context of global conservation priorities even as conservation policies are generally implemented at the local or regional scale. This work aims to identify the regional threats currently impacting species present within the Gulf of Mexico as well as the current polices addressing those threats. Species currently impacted by threats were used to build an ecosystem model to estimate food web dynamics in the Gulf of Mexico. This model is the first of its kind to incorporate data from more than 1500 species occurring in the Gulf including all marine bony shorefishes, marine reptiles, complete clades of select marine invertebrates, marine birds, marine mammals, and chondrichthyans. Comprehensive analyses of these groups are important for an improved understanding of the functioning of the Gulf of Mexico food web and the impact of identified threats on food web dynamics. The identification of current threats and food web dynamics will help to inform conservation policy moving forward. Properly framed conservation efforts are more likely to be widely accepted and successful when there is an improved understanding on how policies can impact stakeholders both economically and through changing practices. Finally, an investigation of the legal frameworks currently recognized in the Gulf of Mexico was done to build an example tri-national framework between the United States, Mexico, and Cuba focusing on current conservation gaps allowing for specific regional conservation concerns to be addressed.