Dielectrophoresis of Gold Nanoparticles

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Description
Dielectrophoretic trapping is a separatory/analytical method that is capable of achieving high levels of analyte differentiation using a combination of electroosmotic flow, electrophoresis, and dielectrophoresis. The form of dielectrophoretic device used in these trials was of a gradient insulator-based design

Dielectrophoretic trapping is a separatory/analytical method that is capable of achieving high levels of analyte differentiation using a combination of electroosmotic flow, electrophoresis, and dielectrophoresis. The form of dielectrophoretic device used in these trials was of a gradient insulator-based design that induced the non-uniform electric fields necessary for dielectrophoretic trapping to occur. Development of such microfluidic devices began in the early 2000s and has produced several successful trials and refinements since then. Improvements have led to the ability of these devices to separate analytes to extremely high degrees of resolution as was demonstrated by the simultaneous separation of antibiotic resistant and antibiotic susceptible strains of bacteria in other experiments. The majority of analytes examined with these microfluidic devices have been biological in nature and on the scale of micrometers in size. The objective of this experiment was to test the lower limit of the device's resolution by attempting to use dielectrophoresis to trap gold nanoparticles via the balancing point between electrophoretic and dielectrophoretic mobilities. Trials successfully captured 10 nm fluorophore tagged gold nanoparticles at a mobility ratio of 6.16 x 1011 V2/m3, 60 nm citrate-capped gold nanoparticles at approximately 3.61 x 1010 V2/m3, and bare 10 nm gold nanoparticle aggregates at both 1.63 x 1010 V2/m3 and 1.68 x 1010 V2/m3. The corresponding voltages that were applied to achieve trapping were -1500 V, -2000 V, and -1500 V respectively. These findings were promising but reproducibility of the results was very low, largely due to matters of contaminants entering the devices and preventing the even, continuous flow of the analyte solution. Refinement of the analytical process should be pursued.
Date Created
2018-12
Agent

Diagnostic and prognostic capacity of serum glycan nodes in different types of cancer

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Description
Glycans are monosaccharide-based heteropolymers that are found covalently attached to many different proteins and lipids and are ubiquitously displayed on the exterior surfaces of cells. Serum glycan composition and structure are well known to be altered in many different types

Glycans are monosaccharide-based heteropolymers that are found covalently attached to many different proteins and lipids and are ubiquitously displayed on the exterior surfaces of cells. Serum glycan composition and structure are well known to be altered in many different types of cancer. In fact, glycans represent a promising but only marginally accessed source of cancer markers. The approach used in this dissertation, which is referred to as “glycan node analysis”, is a molecularly bottom-up approach to plasma/serum (P/S) glycomics based on glycan linkage analysis that captures features such as α2-6 sialylation, β1-6 branching, and core fucosylation as single analytical signals.

The diagnostic utility of this approach as applied to lung cancer patients across all stages as well as prostate, serous ovarian, and pancreatic cancer patients compared to certifiably healthy individuals, nominally healthy individuals and/or risk-matched controls is reported. Markers for terminal fucosylation, α2-6 sialylation, β1-4 branching, β1-6 branching and outer-arm fucosylation were most able to differentiate cases from controls. These markers behaved in a stage-dependent manner in lung cancer as well as other types of cancer. Using a Cox proportional hazards regression model, the ability of these markers to predict progression and survival in lung cancer patients was assessed. In addition, the potential mechanistic role of aberrant P/S glycans in cancer progression is discussed.

Plasma samples from former bladder cancer patients with currently no evidence of disease (NED), non-muscle invasive bladder cancer (NMIBC), and muscle invasive bladder cancer (MIBC) along with certifiably healthy controls were analyzed. Markers for α2-6 sialylation, β1-4 branching, β1-6 branching, and outer-arm fucosylation were able to separate current and former (NED) cases from controls; but NED, NMIBC, and MIBC were not distinguished from one another. Markers for α2-6 sialylation and β1-6 branching were able to predict recurrence from the NED state using a Cox proportional hazards regression model adjusted for age, gender, and time from cancer. These two glycan features were found to be correlated to the concentration of C-reactive protein, a known prognostic marker for bladder cancer, further strengthening the link between inflammation and abnormal plasma protein glycosylation.
Date Created
2018
Agent

Temperature Measurement In Microfluidic Devics

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Description
Microfluidics is an expanding research area for analytical chemistry and the biomedical industry. Microfludic devices have been used for protein and DNA sorting, early detection techniques for cancer and other disease, and a variety of other analytical techniques. Dielectrophoresis is

Microfluidics is an expanding research area for analytical chemistry and the biomedical industry. Microfludic devices have been used for protein and DNA sorting, early detection techniques for cancer and other disease, and a variety of other analytical techniques. Dielectrophoresis is a technique is often used to control particles within microfluidic devices however the non-uniform electric field can affect the interior of the device. In order to expand the applications of microfluidic devices and to make it easier to work with techniques such as dielectrophoresis, it is essential to understand as much as possible about how the internal environment of the device will affect the sample. A significant part of this is being able to non-invasively determine the temperature inside the microfluidic device in the both the channel and reservoir regions. Several other research group have successfully used temperature sensitive dyes and fluorescence to measure the temperature within microfluidic devices so research began with understanding their techniques and trying to optimize them for the chosen microfluidic channel. Results from calibration and reservoir tests show that there is a linear relationship between the temperature of the channel and the ratio between the dyes Rhodamine 110 and Rhodamine B. Results within the channel showed that the calibration may be difficult to apply directly as absorption from the PDMS continues to be a problem but several coatings can be used to improve the results.
Date Created
2013-12
Agent

Differentiation of Staphylococcus epidermidis strains using DC gradient insulator dielectrophoresis

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Description
Bacteria play a vital role in the world ecosystem, more importantly human health and disease. The capability to differentiate and identify these microorganisms serves as an important research objective. In past years, separations-based approaches have served as a way to

Bacteria play a vital role in the world ecosystem, more importantly human health and disease. The capability to differentiate and identify these microorganisms serves as an important research objective. In past years, separations-based approaches have served as a way to observe and identify bacteria based on their characteristics. Gradient insulator dielectrophoresis (g-iDEP) provides benefits in identifying serotypes of a single species with precise separation. Separation of Staphylococcus epidermidis in a single g-iDEP microchannel is conducted exploiting their electrophoretic and electrokinetic properties. The cells were captured and concentrated at gates with interacting forces within the microchannel to clearly distinguish between the two strains. These results provide support for g-iDEP serving as a separating method and, furthermore, future clinical applications.
Date Created
2015-05
Agent