ABSTRACT



Many natural interventions have been effective at lowering postprandial glucose concentrations (PPG) in research trials and, theoretically, should have favorable effects on the prevention and management of T2DM. Natural interventions include vinegar, nuts and exercise. Green tea has been demonstrated to also possessing antiglycemic effects. Thus, green tea, and its most abundant catechin EGCG, are being consumed for its potential health benefits in cancer prevention and in its inhibitory effects on α-amylase. Many studies have found EGCG to inhibit α-amylase an enzyme needed in the breakdown of carbohydrates (CHO). Other studies have looked at EGCG and its potential for lowering PPG concentrations due to its inhibitory effects on α-amylase in both mice and humans. Yet there is no research on Matcha tea specifically. Matcha tea is green tea in powder form; hence, it is consumed in its entirety unlike traditional teas which are steeped in bags. The purpose of this study was to determine whether Macha tea impacts PPG concentrations in healthy adults. Twelve subjects completed this randomized controlled, single blinded, crossover study. On three separate occasions the twelve subjects consumed a bagel and jam with either water, Lipton green tea, or Macha tea. Fasting blood glucose was taken upon their arrival. Once the tea or water and bagel with jam were consumed PPG concentrations were measured every 30 minutes until 120 minutes were reached. Results showed no statistically significant effects on PPG concentrations in either test groups (p=.960). However, this study did not measure EGCG levels in the tea provided. Therefore, further research should be done with known EGCG amounts to see its effects on PPG concentrations to fully rule out its potential.

Epidemiological studies have identified obesity as a risk factor for numerous chronic diseases such as adult onset diabetes, hypertension, and hypercholesterolemia. In both humans and laboratory animals, high-fat diets have been shown to cause obesity. Increases in dietary fat lead to increased energy consumption and, consequently, significant increases in body fat content. CD36 has been implicated in fat perception, preference, and increased consumption, but it is yet to be tested using a behavior paradigm. To study the effect of CD36 on fat taste transmission and fat consumption, four CD36 knockout (experimental) mice and four Black 6 wildtype (control) mice underwent 20 days of fat preference and perception testing. Both groups of mice were exposed to foods with progressively increasing fat content (10%, 12.5%, 15% 17.5%, 20%, 45%) in order to assess the effect of CD36 on fat preference. Afterward, the mice were subjected to an aversive conditioning protocol designed to test the effect of CD36 on fat taste perception; development of a conditioned taste aversion was indicative of ability to taste fat. Especially, knockout mice exhibited diminished preference for and reduced consumption of fat during preference testing and were unable to identify fat taste as the conditioned stimulus during aversive conditioning. A repeated measures ANOVA with Bonferroni correction revealed a significant main effect of group on fat consumption, energy intake, and weight. Linear regression revealed CD36 status to account for a majority of observed variance in fat consumption across both phases of the experiment. These results implicate CD36 in fat taste perception and preference and add to the growing body of evidence suggesting fat as a primary taste.

To date, there have not been any studies in a human population that explore the potential of vinegar ingestion in reducing visceral fat, a common yet serious metabolic disease risk factor. However, previous research in animal models exhibit promising findings, showing that vinegar is effective at reducing visceral fat. This is thought to be due to the activation of AMPK (adenosine monophosphate protein kinase) by acetic acid, the active ingredient in vinegar. The purpose of this study was to identify if this potentially groundbreaking relationship exists in human subjects. Healthy, nonsmoking, sedentary adults between the ages 18-45 y and a waist circumference measurement greater than or equal to 33 inches for women and 38 inches for men were recruited for this study. Twenty-three participants completed this 8-week, parallel arm, randomized control trial that tested the efficacy of red wine vinegar consumption (2 tablespoons red wine vinegar, twice per day, before a meal; providing 3.6 g acetic acid) against a placebo (1 apple cider vinegar pill, twice per day, before a meal; providing 0.0225 g acetic acid) for 8 weeks. Participants were randomized into either the vinegar (VIN) or control (CON) group after being stratified by age, gender, waist circumference, and weight. Results found that the VIN group experienced a 2% decrease in visceral fat (cm3, quantified by a DXA scan), but this change did not differ significantly from that of the CON group (p=0.256). The VIN group also experienced a slight decrease in insulin compared to the CON group, but this change was not significantly different than the control change (p=0.125). However, the change in HOMA-IR trended downward in the VIN group (-16%) as compared to the CON group (+9%) (p=0.079) with a large effect size, 0.153. Other parameters did not show statistically significant results between the groups. Further research is indicated in order to examine the potential of vinegar to reduce visceral fat.

The concept of this thesis is the importance of dietary fiber and how it can be further integrated into the American diet. The adequate intake (AI) of fiber for men and women is thirty-eight and twenty-five grams respectively. I was inspired to focus my research on increasing fiber intake because the typical American consumes fifteen grams of dietary fiber which is well below the AI. The purpose of this project was to inform individuals on the importance of dietary fiber, but also to create and compile recipes which would make it easy for people to increase their intake of dietary fiber. There are two parts to this project: a literature review and a cookbook. The literature review discusses the health benefits of fiber as to how its properties of viscosity and fermentability allow for weight loss, decrease appetite and energy intake, decrease postprandial insulin and glucose levels, impact gut health, lower blood lipid levels in order to protect against atherosclerosis and coronary heart disease, decrease inflammation, and reduce levels of inflammatory marker C-reactive protein. The cookbook provides the ideas for integrating high fiber foods into one's diet. There are three different categories in the cookbook: snacks, lunch and dinner, and breakfast. The snacks and breakfast provide around five grams of fiber per serving, if not more, whereas the lunch and dinner options provide around fifteen grams in a meal. Not only are these recipes high in fiber, but they are also nutrient dense, meaning they provide more than just the listed health benefits in the literature review. Having these recipes and increasing awareness of the benefits which they contain will help individuals to meet the AI of fiber while still enjoying delicious meals.

According to the CDC, diabetes is the 7th leading cause of death in the U.S. and rates are continuing to rise nationally and internationally. Chronically elevated blood glucose levels can lead to type 2 diabetes and other complications. Medications can be used to treat diabetes, but often have side effects. Lifestyle and diet modifications can be just as effective as medications in helping to improve glycemic control, and prevent diabetes or improve the condition in those who have it. Studies have demonstrated that consuming vinegar with carbohydrates can positively impact postprandial glycemia in diabetic and healthy individuals. Continuous vinegar intake with meals may even reduce fasting blood glucose levels. Since vinegar is a primary ingredient in mustard, the purpose of this study was to determine if mustard consumption with a carbohydrate-rich meal (bagel and fruit juice) had an effect on the postprandial blood glucose levels of subjects. The results showed that mustard improved glycemia by 17% when subjects consumed the meal with mustard as opposed to the control. A wide variety of vinegars exists. The defining ingredient in all vinegars is acetic acid, behind the improvement in glycemic response observed with vinegar ingestion. Vinegar-containing foods range from mustard, to vinaigrette dressings, to pickled foods. The benefits of vinegar ingestion with carbohydrates are dose-dependent, meaning that adding even small amounts to meals can help. Making a conscious effort to incorporate these foods into meals, in addition to an overall healthy lifestyle, could provide an additional tool for diabetics and nondiabetics alike to consume carbohydrates in a healthier manner.

Beets have a history of bad reputation for not having the most appetizing qualities compared to other vegetables. Despite the nutritional and health benefits of Beta vulgaris rubra (commonly red beetroot or red beets) the potential of this vegetable has yet to be glorified as compared to i.e. Brassica oleracea var. sabellica (kale), or Chenopodium quinoa (quinoa). When considering this root vegetable as a vehicle for providing your body with a source of dietary nitrate, Beta vulgaris rubra can be classified as a functional food. This work dives deeper into the function of Nitric Oxide (NO) within the human body, and explains the potential benefits of consuming red beets. Followed is a proposal for a cookbook focused on dishes containing this vegetable, as well as a sample of recipes varying from breakfast to dinner to dessert. The amount of nitrate provided by each serving of any of the dishes has not been established, and it is rather a creative attempt to shine positive light on this functional food.

Cases of diet-related illnesses are some of the most common illnesses we witness today. From heart disease to type 2 diabetes, from obesity to hypertension, many of these diseases are easily prevented by lifestyle changes. However, it is much easier to instill healthy habits in a population from the start rather than trying to change habits later, even if one's health depends on it. A pastime as simple as cooking allows us to take responsibility for our own health by (quite literally) taking it into our own hands. This is why I chose to write a cookbook for my honor's thesis. More importantly, this is why I chose to write a cookbook geared towards young children. My target audience is 8-11 years old because that's when I first started to cook, and it's a habit that has kept me well to this day. Within this cookbook, readers will find over 30 (mostly) healthy recipes for breakfast, lunch, dinner, snacks, and desserts, complete with written instructions and photographs to aid with meal preparation. My hope is that, during my career, I will be able to publish this book and distribute it to children who might not have an interest in cooking, but can learn basic cooking skills from my book. If kids get working in the kitchen, they might keep cooking as they grow older, which will help them keep control of their health for the better.

Background. Research suggests that fish oil can be used as an intervention to increase clotting times and reduce the risk of cardiovascular diseases.
Objective. The purpose of this study is to examine the effects of fish oil supplementation on blood coagulation parameters in adults with blood type A (BTA) compared to blood type O (BTO) over an eight-week intervention.
Design. The study was a randomized, double-blind dietary intervention using healthy adults with blood types A or O. A total of 18 participants completed the study. Subjects were randomized into two groups: an experimental group (fish oil) made up of 7 BTO and 4 BTA adults, and a control group (coconut oil) made up of 4 BTO and 3 BTA adults. Non-fasting blood was drawn and analyzed for prothrombin time (PT), activated partial thromboplastin time (PTT), and international normalized ratio (INR) at weeks 0 and 8. A food frequency questionnaire was completed at week 0, and anthropometric data collected at weeks 0 and 8.
Results. Baseline PTT results differed significantly between blood types, 28.1±1.4 seconds and 29.7±1.3 seconds for BTA and BTO respectively (p<0.05). Physical activity differed significantly between the experimental and control group at baseline, 53.9±26.8 METS and 86.0±41.9 METS, respectively (p<0.05). In the Fish oil group, prothrombin time increased for BTA vs. BTO, 0.18±0.29 seconds vs -0.11±0.31 seconds respectively (p<0.10indicating a statistical trend). There were no other differences between groups for the other outcome variables.
Conclusion. Fish oil supplementation prolonged clotting time in BTA adults and may be a useful strategy in this population for reducing cardiovascular disease risk. More research is needed to verify and expand these results.

The aim of this paper is to investigate a few of the primary pillars of the Paleo diet for evidence to either support or refute their efficacy and safety such that a more educated decision can be made by lay-persons who are wishing to make improvements in their overall health via dieting. To accomplish this goal a basic overview of The Paleo Diet (also known to some as the Paleolithic Nutrition Movement) is given based on the writings of Dr. Loren Cordain in his book The Paleo Diet. Next, analyses of a few of the basic characteristics of the diet are presented based on an in-depth literature review that was performed using PubMed (Medline), Cochrane and Google Scholar databases until March of 2015. The findings of this investigation raise concerns with respect to the safety of some of the main principles of the diet such as its high protein, low carbohydrate content that is relies heavily on the consumption of red meat. The current literature on what the diet of the people of the Paleolithic era may have consisted of is also presented in order to shed light on the origins of the diet and see how closely the diet prescribed The Paleo Diet meshes with the most current data on the topic.

The intention of this thesis is to create a cookbook to best serve the needs of all who attend Lions Camp Tatiyee. The cookbook was used as a resource in providing the Lions Camp Tatiyee Kitchen staff with simple, healthy recipes to use in the implementation of their summer menu. This thesis discusses the culmination of the idea, the process of execution, current research concerning the relationship between nutrition and health concerns related to special needs, and achievements and further advancements of my creative project.