Since the 1980s, academics and activists attempting to explain why American evangelicals have supported politicians with controversial environmental track records—from James Watt to James Inhofe—have framed such believers as apocalyptic fatalists content to pillage Creation until Jesus raptures them to safety and destroys the Earth. Today evangelicals maintain higher levels of climate skepticism and lower levels of support for environmental legislation than other religious groups—seemingly confirming the “End Times Apathy Hypothesis.” However, the history of such Rapture-believing, premillennial evangelicals reveals surprisingly sensitive attitudes toward science, nature, and the environmental crisis that stand in stark contrast to popular depictions. Far from promoting anti-science and anti-environmental attitudes, premillennialism has historically encouraged a this-worldly interest in empirical science as believers saw the natural world as a source of revelation and sought to discern the “signs of the times.” It has also offered a flexible theological framework capable of assimilating the most dire findings by environmental scientists and meeting them with hope. Prophecy popularizers such as Billy Graham and Hal Lindsay wrote books which sold in the millions and carried with them the latest findings and predictions by environmental scientists—making them, in effect, some of the most effective science communicators of the twentieth century. Where environmental skepticism has entered evangelicalism, it has been through postmillennial Christian Reconstructionism—a movement deeply opposed to premillennialism—and its promotion of economic cornucopianism, Young Earth Creationism, Christian America historical revisionism, and organizations like the Cornwall Alliance. To make evangelicals into anti-environmentalists, these Reconstructionists first had to unmake them as premillennialists. This interdisciplinary dissertation demonstrates how history and theology can explain evangelicals’ shifting attitudes toward environmentalism. From their early concerns over nuclear testing through their participation in the first Earth Day and up to the eve of the millennium, premillennialism drove evangelicals to take seriously the growing concerns for Creation’s condition and their present attitudes of skepticism and antagonism represent a divergence from this hitherto untold story of apocalyptic environmentalism.

Cyanobacteria and its complex photosynthetic systems have been a prime target for synthetic biologists and their molecular engineering tools for the last couple of decades. However, characterizing meaningful carbon dioxide (CO₂) removal performance has always been a struggle within the field. It is proposed that measuring changes in CO₂ gas concentration within a dynamic system can be accomplished with a simple automated Arduino-powered system. The system employs solenoids in parallel (one for each outlet stream) which are then connected to one large manifold which feeds into a single IR-based CO₂ probe. Since CO₂ probes are expensive, this approach allows for sample multiplexing while remaining affordable. The development of such a system allows for high resolution growth experiments between different strains of cyanobacteria. This approach provides continuous data collection over the entire life cycle of each individual culture, allowing differences in total CO₂ fixation between strains to be readily determined. From a culture of PCC 6803, it was found that the peak mg of CO₂ fixed per day is around 92 mg CO₂/day. In the future, the system can be modified to fit other simple dynamic gas systems, as well as testing similar gas utilization/production capabilities of other organisms.

This dissertation explores the role transportation infrastructure played in regional and community development in northwestern Arizona from 1882 to 1989. Transportation infrastructure undergirds the economic viability and development of most American regions and communities. In northwestern Arizona, following a process familiar throughout the American West, the initial construction of railroad transportation infrastructure fundamentally transformed the area from sparsely populated space to an industrialized region centered on railroad created townsites. Although critical to regional development and growth, U.S. Route 66 was added well after the initial railroad period. In total, regional transformation occurred in four phases: Railroad, Route 66, I-40, and post-bypass. For regional residents, Route 66 was the most important phase transforming railroad created spaces into functional communities. Yet, despite maturing as communities, each of these towns also struggled with the same racial and class divides as the larger nation. From the early railroad period, through WW2, tourism was present in the region, but an ancillary part of the economy focused on visiting environmental attractions like the Grand Canyon. After WW2, it became more important as regional industrialism faded and traffic levels rose on Route 66. However, as long as Route 66 remained a primary highway, tourism retained its focus on the environment. As much as the construction of transportation infrastructure provided initial access to the region and founded towns, the later regional highway and railroad bypasses cut-off many of these communities from the source of their economic livelihood. Regional towns lucky enough to be integrated into the new interstate highway system like Kingman profited and grew; towns bypassed by the interstate and railroad withered and were forced to reinvent themselves to survive. This post-bypass reinvention took the form of a non-environmental focused mythic tourism connected to an emerging national Route 66 nostalgia movement that envisioned the lost Route 66 as representative of a better, more authentic America. The association with the national Route 66 nostalgia myth successfully attracted tourists but came at the cost of regional communities losing a more realistic understanding of their past and becoming disassociated from their previous community identity.

Cyanobacteria have the potential to efficiently produce L-serine, an industrially important amino acid, directly from CO2 and sunlight, which is a more sustainable and inexpensive source of energy as compared to current methods. The research aims to engineer a strain of Cyanobacterium Synechococcus sp. PCC 7002 that increases L-serine production by mutating regulatory mechanisms that natively inhibit its production and encoding an exporter. While an excess of L-serine was not found in the supernatant of the cell cultures, with further fine tuning of the metabolic pathway and culture conditions, high titers of L-serine can be found. With the base strain engineered, the work can be extended and optimized by deleting degradation pathways, tuning gene expression levels, optimizing growth conditions, and investigating the effects of nitrogen supplementation for the strain.

The field of bioprocess engineering has become an increasingly popular route to produce chemicals and fuels in a sustainable fashion. Bioprocessing is an interdisciplinary field that joins chemical engineering, metabolic engineering, and synthetic biology to tackle problems that will arise from the ongoing use of products derived from non-renewable resources. This study will overlook two effective tools that are widely used in the bioprocessing field. The first tool that was studied was strain optimization for biochemical production. This involves genetic manipulation of microbial hosts to create strains that are more efficient at producing the desired products. The second tool that was studied was adaptive laboratory evolution which is used to enhance overall cellular fitness. Enhancing the overall fitness and efficiency of these microbial production factories, allows for innovation and growth in the biochemical industry. Creating sustainable and renewable solutions for both specialty and commodity chemicals.
Strain optimization was specifically studied by enhancing inorganic carbon uptake in synechococcus sp. 7002. It is desired to have both high flux and high affinity transport for the rapid and efficient uptake of HCO3- for enhanced cell growth. The results found that the regulatory gene for carbon transporters in synechococcus genome was successfully deleted. Increasing the toxicity limits of 2-Phenylethanol was done by using adaptive laboratory evolution (ALE). ALE is a widely used practice in biotechnology studies to gain insights on mechanisms of molecular evolution and to better define the functionality of microbial cell factories. It was found that after growing E. coli BW25113 under selective conditions the genome evolved for a higher fitness medium with an increased concentration of 2-Phenylethanol. Overall, two key tools used in bioprocess engineering were successful studied to gain a better insight on the future of biochemical production industry.

In 1916, in the middle of the First World War, Britain developed and deployed the first military tanks on a battlefield, signifying a huge step forward in the combination of mechanization and the military. Tanks represented progress in technical and mechanical terms, but their introduction to military goals and military environments required the men involved to develop immaterial meanings for the tanks. Tactically, tanks required investment from tank commanders and non-tank commanders alike, and incorporating tanks into the everyday routine of the battlefront required men to accommodate these machines into their experiences and perspectives. Reporting the actions of the tanks impelled newspapers and reporters to find ways of presenting the tanks to a civilian audience, tying them to British perspectives on war and granting them positive associations. This thesis sought to identify major concepts and ideas as applied to the British tanks deployed on the Western Front in the First World War, and to better understand how British audiences, both military and civilian, understood and adopted the tank into their understanding of the war. Different audiences had different expectations of the tank, shaped by the environment in which they understood it, and the reaction of those audiences laid the foundation for further development of the tank.

Energiewende refers to the final legislation that sealed the decision to phase out Germany's nuclear power plants prematurely following the Fukushima nuclear disaster. This policy has been in place as of 2011, however, there has been little research done on the comprehensive effects of it within Germany, on German global relationships and obligations. As such, this paper has identified the top areas that have been affected by the legislation as the climate sector, the humanitarian sector and the security sector. In addition to identifying these areas, they have analyzed in the international sphere and this is analysis is used to determine the impact that the German legislation has on political realities in surrounding countries. The impacts are then compared to German obligations, as outlined in numerous treaties from within the three main areas discussed. Not only that, but the effects are also examined within the domestic sphere. The domestic effects are also compared to German obligations from international treaties, while focusing on high priority German issues, such as poverty. This paper ultimately examines the real policy influence that Germany has exhibited as a result of enacting a unique, and ambitious energy policy in a time in which many are still uncertain of the safety, or lack thereof, of nuclear power. The aim is to provide Germany, and countries beginning to question the role of nuclear power, with concurrent solutions to ensure a successful phase out that allows adherence to international and domestic obligations while also illustrating the positive role that nuclear power can play in energy production and policy.