Low Levels of Bisphenol-A Decrease Metabolic Rate and Boldness in Zebrafish, Danio rerio

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Description
Human impact alters the natural environment via multiple pathways, including contamination from pollutants. This human activity may adversely impact an organism’s ability to respond to environmental change. Using Bisphenol-A (BPA), a common environmental contaminant, I examined how exposure affected behavioral

Human impact alters the natural environment via multiple pathways, including contamination from pollutants. This human activity may adversely impact an organism’s ability to respond to environmental change. Using Bisphenol-A (BPA), a common environmental contaminant, I examined how exposure affected behavioral strategies critical for survival in a changing environment. BPA is used during plastic manufacturing, and it enters aquatic systems from wastewater streams; however, it is an endocrine-disruptor that has broad health effects from metabolism to behavior at a wide exposure range. In this study, I specifically tested whether environmentally relevant concentrations of BPA impact maximum metabolic rate and boldness in zebrafish, Danio rerio. I also examined activity level, optomotor response, body mass, and standard length to see if I can mechanistically explain any underlying changes caused by BPA. I treated groups of adult zebrafish for 7 days and exposed them to either 0.1% dimethyl sulfoxide (DMSO, control), a low environmentally relevant concentration of BPA (0.02 mg/L), or a 1-fold higher BPA concentration (0.2 mg/L). I found that the low exposure group experienced a decrease in maximum metabolic rate and the high exposure group showed a decrease in boldness. In other words, these changes in metabolism were not dosage dependent while the boldness results were dosage dependent. BPA had no effects on optomotor response, body mass, standard length or activity level. These results suggest that no level of BPA is safe, environmentally relevant concentrations are having an effect on adult organisms’ behavior and health that could affect their survival.
Date Created
2021
Agent

Sentinels of the Sea: Marine Mammal Behavioral Responses to Environmental Change

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Description
This research investigates how two potential sentinel species (the Galápagos Sea Lion (Zalophus wollebaeki) and the Guiana Dolphin (Sotalia guianensis)) respond to environmental factors, at both the large-scale and fine-scale levels. Sentinel species, defined as organisms able to respond to

This research investigates how two potential sentinel species (the Galápagos Sea Lion (Zalophus wollebaeki) and the Guiana Dolphin (Sotalia guianensis)) respond to environmental factors, at both the large-scale and fine-scale levels. Sentinel species, defined as organisms able to respond to ecosystem variability and/or change in a timely and measurable way to nowcast or forecast otherwise unobserved environmental changes, can help mitigate or even avoid changes deleterious to both wildlife and human communities. Using two long-term datasets and a suite of respective social metrics and environmental factors, I analyzed potential external influences on these two species’ behavioral ecology. My overall findings suggest that apex marine mammals respond differently to their surroundings at large-scale vs. fine-scale, and highlight the importance of including a range of environmental factors that include anthropogenic effects. Galápagos Sea Lions specifically respond to thermoregulation-linked factors, such as substrate temperature, and anthropogenic factors such as human presence and activity type. Guiana Dolphin social metrics are significantly related with traits linked to environmental water quality, water transparency. I expand on the sentinel implications of these results and introduce sample methodology and results for sentinel species based on the Guiana Dolphin case study.
Date Created
2021
Agent

The role of skill in determining dominance in the virile crayfish, Faxonius virilis

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Description

Studies of animal contests often focus solely on a single static measurement of fighting ability, such as the size or the strength of the individual. However, recent studies have highlighted the importance of individual variation in the dynamic behaviors used

Studies of animal contests often focus solely on a single static measurement of fighting ability, such as the size or the strength of the individual. However, recent studies have highlighted the importance of individual variation in the dynamic behaviors used during a fight, such as, assessment strategies, decision making, and fine motor control, as being strong predictors of the outcome of aggression. Here, I combined morphological and behavioral data to discover how these features interact during aggressing interactions in male virile crayfish, Faxonius virilis. I predicted that individual variation in behavioral skill for decision making (i.e., number of strikes thrown), would determine the outcome of contest success in addition to morphological measurements (e.g. body size, relative claw size). To evaluate this prediction, I filmed staged territorial interactions between male F. virilis and later analyzed trial behaviors (e.g. strike, pinches, and bout time) and aggressive outcomes. I found very little support for skill to predict win/loss outcome in trials. Instead, I found that larger crayfish engaged in aggression for longer compared to smaller crayfish, but that larger crayfish did not engage in a greater number of claw strikes or pinches when controlling for encounter duration. Future studies should continue to investigate the role of skill, by using finer-scale techniques such as 3D tracking software, which could track advanced measurements (e.g. speed, angle, and movement efficiency). Such studies would provide a more comprehensive understanding of the relative influence of fighting skill technique on territorial contests.

Date Created
2021-05
Agent

Behavioral Basis of Sensorimotor Control and Learning

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Description

Motor learning is the process of improving task execution according to some measure of performance. This can be divided into skill learning, a model-free process, and adaptation, a model-based process. Prior studies have indicated that adaptation results from two complementary

Motor learning is the process of improving task execution according to some measure of performance. This can be divided into skill learning, a model-free process, and adaptation, a model-based process. Prior studies have indicated that adaptation results from two complementary learning systems with parallel organization. This report attempted to answer the question of whether a similar interaction leads to savings, a model-free process that is described as faster relearning when experiencing something familiar. This was tested in a two-week reaching task conducted on a robotic arm capable of perturbing movements. The task was designed so that the two sessions differed in their history of errors. By measuring the change in the learning rate, the savings was determined at various points. The results showed that the history of errors successfully modulated savings. Thus, this supports the notion that the two complementary systems interact to develop savings. Additionally, this report was part of a larger study that will explore the organizational structure of the complementary systems as well as the neural basis of this motor learning.

Date Created
2021-05
Agent

Chemical Cues Impact Zebrafish Response to Visual Cues

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Description

Animals encounter information from different resources simultaneously, integrating input from multiple sensory systems before responding behaviorally. When different cues interact with one another, they may enhance, diminish, or have no impact on their responses. In this project, we test how

Animals encounter information from different resources simultaneously, integrating input from multiple sensory systems before responding behaviorally. When different cues interact with one another, they may enhance, diminish, or have no impact on their responses. In this project, we test how the presence of chemical cues affect the perception of visual cues. Zebrafish (Danio rerio) often use both chemical cues and visual cues to communicate with shoal mates, to assess predation risk, and to locate food. For example, zebrafish rely on both olfactory cues and visual cues for kin recognition, and they frequently use both chemical and visual cues to search for and to capture prey. In zebrafish, the terminal nerve (TN) constitutes the olfacto-visual centrifugal pathway and connects the olfactory bulb with the retina, thus allowing olfactory perception also to activate visual receptors. Past studies have found that the presence of an olfactory cue can modulate visual sensitivity in zebrafish through the terminal nerve pathway. Alternatively, given that zebrafish are highly social, the presence of social chemical cues may distract individuals from responding to other visual cues, such as food and predator visual cues. Foraging and predator chemical cues, including chemical food cues and alarm cues, may also distract individuals from responding to non-essential visual cues. Here, we test whether the response to a visual cue either increases or decreases when presented in concert with alanine, an amino acid that represents the olfactory cues of zebrafish prey. We found that the presence of chemical cues did not affect whether zebrafish responded to visual cues, but that the fish took longer to respond to visual cues when chemical cues were also present. These findings suggest that different aspects of behavior could be affected by the interaction between sensory modalities. We also found that this impact of delayed response was significant only when the visual cue<br/>was weak compared to the strength of the chemical cue, suggesting that the salience of interacting cues may also have an influence on determining the outcomes of the interactions. Overall, the interactive effects of chemicals on an animal’s response to visual cues may also have wide-ranging impacts on behavior including foraging, mating, and evading predators, and the interaction of cues may affect different aspects of the same behavior.

Date Created
2021-05
Agent

In Andy's Shoes: A Children's Book and Manual on the Expression of Autism Spectrum Disorder

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Description

The purpose of this project was to research the expression of Autism Spectrum Disorder in children and create a children’s book that can help the peers of individuals with a diagnosis to understand what the disorder entails and potentially gain

The purpose of this project was to research the expression of Autism Spectrum Disorder in children and create a children’s book that can help the peers of individuals with a diagnosis to understand what the disorder entails and potentially gain a new sense of empathy for peers of all levels of physical and mental abilities. The research component includes interviews with individuals deemed knowledgeable about ASD, including occupational therapists, behavioral analysts, and parents, as well as a literature review of research studies on the expression of Autism in children. This written portion of the project may also serve as a manual for individuals who have little to no knowledge of ASD, as it dives deeper into the content of the book and research, while remaining easily understandable and clear to those without any prior knowledge or experience with ASD. It could prove especially useful for those in professions that come into contact with individuals with Autism, but do not necessarily require psychology courses or training as a prerequisite for the role, such as teachers and some health professionals.

Date Created
2021-05
Agent

Do Arizona Charter Schools Rely on Exclusionary Discipline Practices?

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Description

Families of students with disabilities and those who are culturally and linguistically diverse (CLD), are looking for better educational opportunities. Charter schools offer promise as they were designed to promote student learning with limited control from the state. Charter schools

Families of students with disabilities and those who are culturally and linguistically diverse (CLD), are looking for better educational opportunities. Charter schools offer promise as they were designed to promote student learning with limited control from the state. Charter schools though, have been criticized for relying on exclusionary discipline policies that affect CLD students and students with disabilities disproportionately. This study was designed to understand how Arizona charter schools use exclusionary discipline practices, with a focus on students with disabilities and CLD students. Two participants, a fourth grade and fifth grade teacher from a Phoenix metropolis charter school completed surveys and interviews where they answered questions about their classroom and their school’s discipline policies. Teachers were asked how they have adapted and administered classroom discipline policies and to what extent have positive behavioral strategies been implemented in an online setting due to the COVID-19 pandemic when schools transitioned to virtual learning. The results showed that in a virtual setting, teachers retained the practice of removing students from the “classroom”, expectations had to be modified to meet the needs of the new environment, and the school counselor served in conflicting roles. The findings suggested that charter schools and teachers may be transferring and adapting their reliance on exclusionary discipline practices even for an online setting with classrooms that have students with disabilities and those who are CLD.

Date Created
2021-05
Agent

Automobile Idling Reduction Program

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Description
Description
By avoiding vehicle idling for three minutes every day of the year can reduce 1.4 million metric tons annually, which is equivalent to taking 320,000 cars off the road for the entire year (Canada.ca, 2016). The Automobile Idle Reduction Program

Description
By avoiding vehicle idling for three minutes every day of the year can reduce 1.4 million metric tons annually, which is equivalent to taking 320,000 cars off the road for the entire year (Canada.ca, 2016). The Automobile Idle Reduction Program (AIRP) is an outreach initiative to prevent carbon emissions from being released into the air by automobiles idling in Maricopa County. The initiative establishes a campaign to promote behavioral changes that target high idling industries: freight and delivery, schools and drive- thru facilities.

Background
Globally, carbon emissions negatively alter the air we breathe and is a leading cause in climate change. These problems adversely affect the global environment and human health. Additionally, they have cancer causing agents in the particulate matter. Unfortunately, over the years, Maricopa County has failed to meet air quality standards for particulate matter pollution which effects the health of residents. By not meeting the air quality standards, Maricopa County can receive sanctions and the Environmental Protection Agency can reject Arizona’s State Implementation Plan. This looming threat can financially impinge the economy of Maricopa County, potentially costing taxpayers a substantial increase in taxes.

Strategy and Solution
To battle the creation of carbon emissions and particulate matter, AIRP has developed a strategy for each industry. In partnership with the Maricopa County Air Quality Department, AIRP will introduce the freight and delivery companies to the Diesel Emission Reduction Act (DERA) Grant promotion to facilitate and fiscally assist with changing older diesel engines into higher efficiency engines that burn cleaner. Provide educators a fifth to eighth grade state approved education program to teach students the importance of vehicle idling reduction at no cost. And work with community organizations to offer a discount at their stores for those patrons who choose to turn their engine off and order inside, rather than idling in the drive-thru facilities. The campaign will market the interest of AIRP to the general public through purposefully placed billboards, light rail wraps, social media pushes, handouts and vinyl stickers.
Date Created
2020-05-13
Agent

Discrepancies in the Morris water maze versus the IntelliCage in the APP/PS1 mouse model of Alzheimer’s disease; a sex-based examination

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Description
Dementia is a collective term used to describe symptoms of cognitive impairment in learning and memory. The most prevalent form of dementia is Alzheimer’s disease (AD). In order to understand the pathological mechanisms associated with AD, animal models have been

Dementia is a collective term used to describe symptoms of cognitive impairment in learning and memory. The most prevalent form of dementia is Alzheimer’s disease (AD). In order to understand the pathological mechanisms associated with AD, animal models have been created. These various mouse models replicate the pathology found in humans with AD. As a consequence of the fact that this disease impairs cognitive abilities in humans, testing apparatuses have been developed to measure impaired cognition in animal models. One of the most common behavioral apparatuses that has been in use for nearly 40 years is the Morris water maze (MWM). In the MWM, animals are tasked to find a hidden platform in a pool of water and thereby are subjected to stress that can unpredictably influence cognitive performance. In an attempt to circumvent such issues, the IntelliCage was designed to remove the external stress of the human experimenter and provide a social environment during task assessment which is fully automated and programable. Additionally, the motivation is water consumption, which is less stressful than escaping a pool. This study examined the difference in performance of male and female cohorts of APP/PS1 and non-transgenic (NonTg) mice in both the MWM and the IntelliCage. Initially, 12-month-old male and female APP/PS1 and NonTg mice were tested in the hippocampal-dependent MWM maze for five days. Next, animals were moved to the IntelliCage and underwent 39 days of testing to assess prefrontal cortical and hippocampal function. The results of this experiment showed significant sex differences in task performance, but inconsistency between the two testing paradigms. Notably, males performed significantly better in the MWM, which is consistent with prior research. Interestingly however, APP/PS1 females showed higher Amyloid-β plaque load and performed significantly better in the more complex tasks of the IntelliCage. This suggests that Aβ plaque load may not directly contribute to cognitive deficits, which is consistent with recent reports in humans with AD. Collectively, these results should inform scientists about the caveats of behavioral paradigms and will aid in determining translation to the human condition.
Date Created
2020-05
Agent

A Virtual Sholl Analysis of the Neuronal Morphological Impact of Stress in Rats

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Description
Stress activates physiological systems within the body to protect oneself against the potential harmful effects of enduring long-term stress. Past studies have shown that structures involved in timing are implicated in a number of psychological disorders and further are sensitive

Stress activates physiological systems within the body to protect oneself against the potential harmful effects of enduring long-term stress. Past studies have shown that structures involved in timing are implicated in a number of psychological disorders and further are sensitive to stress. In this experiment, Sprague Dawley rats are trained to perform a perspective timing task and are then exposed to twice-daily chronic variable stress for 21 days. Behavioral data are collected, followed by post-mortem tissue analysis of the PFC, hippocampus, and striatum. This study aims to examine the morphological changes in key brain regions such as the hippocampus that appear to be involved in interval timing. Additionally, this study aims to confirm that dendritic complexity in the hippocampus produces consistent data using a classic Sholl analysis versus using a virtual image-stacking software, Neurostackr. The results of this study demonstrate that the expected Gaussian graph produced from a classic Sholl analysis was produced from both a long-shaft and short-shaft neuron found in the hippocampus using the virtual technology. These findings verify that a virtual image-stacking software and Sholl analysis will suffice in place of the traditional method of hand traced neurons on a transparent sheet with concentric circles to count bifurcation points. This virtual method ultimately reduces cost, improves timeliness of data collection, and eliminates some of the subjectivity of human error.
Date Created
2020-05
Agent