The physiological phenomenon of sensing temperature is detected by transient

receptor (TRP) ion channels, which are pore forming proteins that reside in the

membrane bilayer. The cold and hot sensing TRP channels named TRPV1 and TRPM8

respectively, can be modulated by diverse stimuli and are finely tuned by proteins and

lipids. PIRT (phosphoinositide interacting regulator of TRP channels) is a small

membrane protein that modifies TRPV1 responses to heat and TRPM8 responses to cold.

In this dissertation, the first direct measurements between PIRT and TRPM8 are

quantified with nuclear magnetic resonance and microscale thermophoresis. Using

Rosetta computational biology, TRPM8 is modeled with a regulatory, and functionally

essential, lipid named PIP2. Furthermore, a PIRT ligand screen identified several novel

small molecular binders for PIRT as well a protein named calmodulin. The ligand

screening results implicate PIRT in diverse physiological functions. Additionally, sparse

NMR data and state of the art Rosetta protocols were used to experimentally guide PIRT

structure predictions. Finally, the mechanism of thermosensing from the evolutionarily

conserved sensing domain of TRPV1 was investigated using NMR. The body of work

presented herein advances the understanding of thermosensing and TRP channel function

with TRP channel regulatory implications for PIRT.