Human locomotion is an essential function that enables individuals to lead healthy, independent lives. One important feature of natural walking is the capacity to transition across varying surfaces, enabling an individual to traverse complex terrains while maintaining balance. There has been extensive work regarding improving prostheses' performance in changing walking conditions, but there is still a need to address the transition from rigid to compliant or dynamic surfaces, such as the transition from pavement to long grass or soft sand. This research aims to investigate the mechanisms involved such transitions and identify potential indicators of the anticipated change that can be applied to the control of a powered ankle prosthetic to reduce falls and improve stability in lower-limb amputees in a wider range of walking environments. A series of human subject experiments were conducted using the Variable Stiffness Treadmill (VST) to control walking surface compliance while gait kinematics and muscular activation data were collected from three healthy, nondisabled subjects. Specifically, the kinematics and electromyography (EMG) profiles of the gait cycles immediately preceding and following an expected change in surface compliance were compared to that of normal, rigid surface walking. While the results do not indicate statistical differences in the EMG profiles between the two modes of walking, the muscle activation appears to be qualitatively different from inspection of the data. Additionally, there were promising statistically significant changes in joint angles, especially in observed increases in hip flexion during the swing phases both before and during an expected change in surface. Decreases in ankle flexion immediately before heel strike on the perturbed leg were also observed to occur simultaneously with decreases in tibialis anterior (TA) muscle activation, which encourages additional research investigating potential changes in EMG profiles. Ultimately, more work should be done to make strong conclusions about potential indicators of walking surface transitions, but this research demonstrates the potential of EMG and kinematic data to be used in the control of a powered ankle prosthetic.