My research focuses on studying the interaction between spatiotemporally encoded electric field (EF) and living cells and biomolecules. In this thesis, I report two projects that I have been working on to address these questions. My first project studies the EF modulation of the extracellular-signal-regulated kinase (ERK) pathway. I demonstrated modulation of ERK activities using alternative current (AC) EFs in a new frequency range applied through high-k dielectric passivated microelectrodes with single-cell resolution without electrochemical process induced by the EF stimulation. Further experiments pinpointed a mechanism of phosphorylation site of epidermal growth factor (EGF) receptor to activate the EGFR-ERK pathway that is independent of EGF. AC EFs provide a new strategy to precisely control the dynamics of ERK activation, which may serve as a powerful platform for control of cell behaviors with implications in wide range of biomedical applications. In the second project, I used solid-state nanopore system as the base platform for single molecule experiments, and developed a scalable bottom-up process to construct planar nanopore devices with self-aligned transverse tunneling junctions, all embedded on a nanofluidic chip, based on feedback-controlled reversible electrochemical deposition in a confined nanoscale space. I demonstrated the first simultaneous detection of translocating DNA molecules from both the ionic channel and the tunneling junction with very high yield. Meanwhile, the signal amplitudes from the tunneling junction are unexpectedly high, indicating that these signals are probably dominated by transient currents associated with the fast motion of charged molecules between the transverse electrodes. This new platform provides the flexibility and reproducibility required to study quantum-tunneling-based DNA detection and sequencing. In summary, I have developed two platforms that engineer heterogenous EF at different length scales to modulate live cells and single biomolecules. My results suggest that the charges and dipoles of biomolecules can be electrostatically manipulated to regulate physiological responses and to push detection resolution to single molecule level. Nevertheless, there are still many interesting questions remain, such as the molecular mechanism of EF-protein interaction and tunneling signal extraction. These will be the topics for future investigations.

Lighting systems and air-conditioning systems are two of the largest energy consuming end-uses in buildings. Lighting control in smart buildings and homes can be automated by having computer controlled lights and window blinds along with illumination sensors that are distributed in the building, while temperature control can be automated by having computer controlled air-conditioning systems. However, programming actuators in a large-scale environment for buildings and homes can be time consuming and expensive. This dissertation presents an approach that algorithmically sets up the control system that can automate any building without requiring custom programming. This is achieved by imbibing the system self calibrating and self learning abilities.

For lighting control, the dissertation describes how the problem is non-deterministic polynomial-time hard(NP-Hard) but can be resolved by heuristics. The resulting system controls blinds to ensure uniform lighting and also adds artificial illumination to ensure light coverage remains adequate at all times of the day, while adjusting for weather and seasons. In the absence of daylight, the system resorts to artificial lighting.

For temperature control, the dissertation describes how the temperature control problem is modeled using convex quadratic programming. The impact of every air conditioner on each sensor at a particular time is learnt using a linear regression model. The resulting system controls air-conditioning equipments to ensure the maintenance of user comfort and low cost of energy consumptions. The system can be deployed in large scale environments. It can accept multiple target setpoints at a time, which improves the flexibility and efficiency of cooling systems requiring temperature control.

The methods proposed work as generic control algorithms and are not preprogrammed for a particular place or building. The feasibility, adaptivity and scalability features of the system have been validated through various actual and simulated experiments.