An Investigation of the Formation, Function, and Future of Extrachromosomal Circular DNA
Description
Extrachromosomal circular DNA (eccDNA) has been identified in a broad range of eukaryotes and have been shown to carry genes and regulatory sequences. Additionally, they can amplify within a cell by autonomous replication or reintegration into the genome, effectively influencing copy number in cells. This has significant implications for cancer, where oncogenes are frequently amplified on eccDNA. However, little is known about the exact molecular mechanisms governing eccDNA functionality. To this end, we constructed a fluorescent reporter at an eccDNA-prone locus of the yeast genome, CUP1. It is our hope that this reporter will contribute to a better understanding of eccDNA formation and amplification within a cell.
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2021-05
Agent
- Author (aut): Keal, Tula Ann
- Thesis director: Wang, Xiao
- Committee member: Tian, Xiaojun
- Contributor (ctb): School of Life Sciences
- Contributor (ctb): School of Life Sciences
- Contributor (ctb): Barrett, The Honors College