Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp. Mutant Induced by Heavy Carbon Ions
Description
The unicellular green microalga Desmodesmus sp. S1 can produce more than 50% total lipid of cell dry weight under high light and nitrogen-limitation conditions. After irradiation by heavy 12C6+ ion beam of 10, 30, 60, 90 or 120 Gy, followed by screening of resulting mutants on 24-well microplates, more than 500 mutants were obtained. One of those, named D90G-19, exhibited lipid productivity of 0.298 g L-1⋅d-1, 20.6% higher than wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis under stress. This work demonstrated that heavy-ion irradiation combined with high-throughput screening is an effective means for trait improvement. The resulting mutant D90G-19 may be used for enhanced lipid production.
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2013-04-09
Agent
- Author (aut): Hu, Guangrong
- Author (aut): Fan, Yong
- Author (aut): Zhang, Lei
- Author (aut): Yuan, Cheng
- Author (aut): Wang, Jufang
- Author (aut): Hu, Qiang
- Author (aut): Li, Fuli
- Contributor (ctb): Julie Ann Wrigley Global Institute of Sustainability