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The unicellular green microalga Desmodesmus sp. S1 can produce more than 50% total lipid of cell dry weight under high light and nitrogen-limitation conditions. After irradiation by heavy 12C6+ ion beam of 10, 30, 60, 90 or 120 Gy, followed

The unicellular green microalga Desmodesmus sp. S1 can produce more than 50% total lipid of cell dry weight under high light and nitrogen-limitation conditions. After irradiation by heavy 12C6+ ion beam of 10, 30, 60, 90 or 120 Gy, followed by screening of resulting mutants on 24-well microplates, more than 500 mutants were obtained. One of those, named D90G-19, exhibited lipid productivity of 0.298 g L-1⋅d-1, 20.6% higher than wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis under stress. This work demonstrated that heavy-ion irradiation combined with high-throughput screening is an effective means for trait improvement. The resulting mutant D90G-19 may be used for enhanced lipid production.

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    Title
    • Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp. Mutant Induced by Heavy Carbon Ions
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    Date Created
    2013-04-09
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  • Text
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    Identifier
    • Digital object identifier: 10.1371/journal.pone.0060700
    • Identifier Type
      International standard serial number
      Identifier Value
      1045-3830
    • Identifier Type
      International standard serial number
      Identifier Value
      1939-1560
    Note
    • The article is published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0060700

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    Hu, G., Fan, Y., Zhang, L., Yuan, C., Wang, J., Li, W., . . . Li, F. (2013). Enhanced Lipid Productivity and Photosynthesis Efficiency in a Desmodesmus sp. Mutant Induced by Heavy Carbon Ions. PLoS ONE, 8(4). doi:10.1371/journal.pone.0060700

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