Autism spectrum disorder (ASD) currently lacks a biological diagnostic test, ongoing research is being conducted to develop a urine biomarker test for autism. Researchers are investigating possible anions, such as sulfur-based anions, as a biomarker for autism. Although studies have not measured the quantification of sulfate-based anions within a biospecimen while using Ion Chromatography (IC) for a 24-hour period. Research studies on autism biomarker development could greatly benefit by investigating and quantifying sulfur-based anions such as sulfate, sulfide, sulfite, or thiosulfate. Our research investigated the quantifications of anions through the analysis of biospecimens across 24-hours in an IC. The results of our research indicate that sulfate fluctuates the least and was consistently read by the IC at each time point across 24 hours whereas the other anions of interest presented greater fluctuations and were not detected at each time point across the 24 hours under the conditions tested.