Diffusion weighted imaging utilizes magnetic resonance imaging to capture white matter tracts in the brain. Many studies have utilized this technique to measure white matter structures looking for evidence of anatomical and physiological differences in Autism Spectrum Disorder (ASD). Parkinsonian-like symptoms have been documented and self-reported in aging autistic individuals, opening up questions about a possible link between ASD and an increased risk of Parkinson’s Disease. Utilizing MRtrix3, an image processing proof-of-concept pipeline was developed for the Autism and Brain Aging (ABA) lab to generate and visualize the brain’s structural connectivity network in these populations of interest. The pipeline provides the opportunity for white matter tractography analysis in the lab’s Aging in Autism study.