DC-DC converters are widely employed to interface one voltage level with another through step-up or step-down operation. In recent years, step-up DC-DC converters have been a key component in harnessing energy through renewable sources by providing an interface to integrate low voltage systems to DC-AC converters or microgrids. They find increasing applications in battery and fuel cell electric vehicles which can benefit from high and variable DC link voltage. It is important to optimize these converters for higher efficiency while achieving high gain and high power density. Non-isolated DC-DC converters are an attractive option due to the reduced complexity of magnetic design, smaller size, and lower cost. However, in these topologies, achieving a very high gain along with high efficiency has been a challenge. This work encompasses different non-isolated high gain DC-DC converters for electric vehicle and renewable energy applications. The converter topologies proposed in this work can easily achieve a conversion ratio above 20 with lower voltage and current stress across devices. For applications requiring wide input or output voltage range, different control schemes, as well as modified converter configurations, are proposed. Moreover, the converter performance is optimized by employing wide band-gap devices-based hardware prototypes. It enables higher switching frequency operation with lower switching losses. In recent times, multiple soft-switching techniques have been introduced which enable higher switching frequency operation by minimizing the switching loss. This work also discusses different soft-switching mechanisms for the high conversion ratio converter and the proposed mechanism improves the converter efficiency significantly while reducing the inductor size. Further, a novel electric vehicle traction architecture with low voltage battery and multi-input high gain DC-DC converter is introduced in this work. The proposed architecture with multiple 48 V battery packs and integrated, multi-input, high conversion ratio DC-DC converters, can reduce the maximum voltage in the vehicle during emergencies to 48 V, mitigate cell balancing issues in battery, and provide a wide variable DC link voltage. The implementation of high conversion ratio converter in multiple configurations for the proposed architecture has been discussed in detail and the proposed converter operation is validated experimentally through a scaled hardware prototype.