Every year an alarming number of deaths for the Black community are a result of disparities and inequalities in health outcomes. While literature has largely focused on social determinants of heath (e.g., economic, environmental, biological, and behavioral structures) as contributing factors to disparate health outcomes for Black people, literature on medical mistrust has been on the rise. Medical mistrust is defined as the belief that health care entities and providers act against a patient's best interest and well-being, and is associated with lower rates of service utilization, inadequate management of health conditions, lower levels of involvement in research, and treatment nonadherence. Only recently has patient-centered care been examined as a construct that may reduce the negative effects of medical mistrust. This study examined Black identifying patients (N = 174) across gender and their reported levels of medical mistrust, and if the perception of a patient-centered health care environment would moderate the association. The findings indicated that Black females, compared to Black males, endorsed higher levels of medical mistrust that may be indicative of intersectional influences. While there were significant effects of gender and perceived patient-centered care on medical mistrust, perceived patient-centered care was not found to significantly moderate the relationship between gender identity and medical mistrust. This may be indicative of the varying degrees of medical maladies that may be stronger determinants of perceived patient-centered care, despite gender or other demographic characteristics. Implications for practice and future research on the intersectional influences on medical mistrust and perceived patient-centered care in the Black communities are discussed.