This dissertation integrates concepts from three bodies of literature: police use of force, neighborhood/ecological influence on police, and police culture. Prior research has generally found that neighborhood context affects police use of force. While scholars have applied social disorganization theory to understand why neighborhood context might influence use of force, much of this theorizing and subsequent empirical research has focused exclusively on structural characteristics of an area, such as economic disadvantage, crime rates, and population demographics. This exclusive focus has occurred despite the fact that culture was once an important component of social disorganization theory in addition to structural factors. Moreover, the majority of the theorizing and subsequent research on police culture has neglected the potential influence that neighborhood context might have on officers’ occupational outlooks. The purpose of this dissertation is to merge the structural and cultural elements of social disorganization theory in order to shed light on the development and maintenance of police officer culture as well as to further specify the relationship between neighborhood context and police use of force. Using data from the Project on Policing Neighborhoods (POPN), I address three interrelated research questions: 1) does variation of structural characteristics at the patrol beat level, such as concentrated disadvantage, homicide rates, and the percentage of minority citizens, predict how an officer views his/her occupational outlook (i.e., culture)?; 2) do officers who work in the same patrol beats share a similar occupational outlook (i.e., culture) or is there variation?; and 3) does the inclusion of police culture at the officer level moderate the relationship between patrol beat context and police use of force? Findings suggest that a patrol beat’s degree of concentrated disadvantage and homicide rate slightly influence officer culture at the individual level. Results show mixed evidence of a patrol beat culture. There is little support for the idea that characteristics of the patrol beat and individual officer culture interact to influence police use of force. I conclude with a detailed discussion of the methodological, theoretical, and policy implications as well as limitations and directions for future research.