Current research on anti-gay attitudes has focused heavily on heterosexuals versus

non-heterosexuals, with very little research delving into the differences within these “non-heterosexual” groups. The author conducted an exploratory analysis of how the intersectional effect of gender and sexual orientation affect perceptions of target groups’ gender and sexuality, which in turn might explain different levels of prejudice toward LGBT subgroups. Based on previous studies, the author hypothesized that participants would believe that a gay male has a more fixed sexuality than a lesbian, leading in turn to higher levels of moral outrage. This study further aims to extend the literature to perceptions of bisexual and transgender individuals by testing competing hypotheses. Participants might feel less moral outrage toward these groups than other LGBT subgroups because they believe their sexuality is even less fixed than lesbians’. Alternatively, participants might feel more moral outrage toward bisexual and transgender targets (versus other LGBT groups) because of the uncomfortable feeling of uncertainty about these groups’ sexuality and/or gender. Overall, participants demonstrated an interactive effect of gender and sexuality on factors including perceived sexual orientation, perceived biological sex, perceived gender identity, perceived sexual fixedness, and moral outrage rather than gender having a main effect on perceptions of gender and sexual orientation having a main effect on perceptions of sexuality. Furthermore, perceptions of sexual fixedness mediated the effect of gender on moral outrage for heterosexual target groups, but not gay targets. Gender certainty mediated the effect of gender on moral outrage for pre-op transgender target groups, but not heterosexuals. This work is important to inform future research on the topics of the intersection of sexuality and gender, especially to extend the limited literature on perceptions of bisexual and transgender individuals.