This study examined the relationships between the amount of physical activity engagement and two sets of health-related tests: measures of physical fitness (abdominal curl-ups, push-ups, handgrip strength, hip flexibility, and cardiorespiratory fitness) as well as measures of motor skill performance (kicking, throwing, jumping, hopping, running, and standing from a supine position) in mid-life women (ages 45-65). Physical activity engagement was assessed using 7-day accelerometer readings and the Stanford Brief Activity Survey. Motor skill performance was assessed using scores of maximum kicking, throwing, jumping, hopping, and running speeds and maximum jumping distance. Physical fitness was assessed using scores of maximum abdominal curl-ups, push-ups, handgrip strength, hip flexibility, and cardiorespiratory fitness. Results suggest that regular participation in moderate lifestyle, walking, and vigorous physical activity are related to better performances in curl-ups, push-ups, cardiorespiratory fitness on a submaximal treadmill test, kicking, throwing, and transitioning from a supine position to standing. These data represent the feasibility of selected motor skills and physical fitness tests for mid-life women and suggest that a relationship may be present between selected motor skills and health-related physical fitness measures and physical activity.