Determining Magnesium Metal Affinity of Alpha L- Id in pH 5
Description
Integrin is a protein in cells that manage cell adhesion. They are crucial to the biochemical functions of cells. L 2 is one type of integrin. Its I domain is responsible for ligand binding. Scientists understand how Alpha L I domain binds Mg2+ at a pH of 7 but not in acidic environments. Knowing the specificity of integrin at a lower pH is important because when tissues become inflamed, they release acidic compounds. We have cloned, expressed, and purified L I-domain and using NMR analysis, we determined that wild type Alpha L I domain does not bind to Mg2+ at a pH of 5.
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2017-05
Agent
- Author (aut): ALAM, RAHAT
- Thesis director: Wang, Xu
- Committee member: Podolnikova, Nataly
- Contributor (ctb): School of Molecular Sciences
- Contributor (ctb): Barrett, The Honors College