The termite Zootermopsis nevadensis nuttingi, which is located in coastal forests of the western United States, plays an important ecological role in the breakdown and digestion of wood. Vital to this role are symbiotic protists residing in the termite's hindgut. Five protist genera of varying size and morphology make up this gut community. Despite years of study on this termite species, little was known about the spatial organization of the protist community within Zootermopsis nevadensis nuttingi. To resolve this issue, a study was conducted in which the distribution of protist genera among gut segments was observed and elucidated. This was done by separating hindgut segments, then counting the protists using a hemocytometer at a magnification of 200x. 60 segments from 20 termites were examined, and the total number of protists counted was 69,560. Images were also taken using a scanning electron microscope. Statistically significant, distinct distribution patterns were found for Trichonympha, Trichomitopsis and Streblomastix, while the small genera of Hexamastix and Tricercomitus appeared to have no special distribution. Trichomitopsis was more abundant in the posterior hindgut, Streblomastix was more abundant anteriorly, while the distribution of Trichonympha varied by colony. Hexamastix and Tricercomitus make up a large majority of the protists observed in any segment, followed by Streblomastix, Trichomitopsis and Trichonympha. Understanding the distribution of different protists within the hindgut may improve our understanding of the ecological relationships among protists as well as their individual roles in lignocellulose digestion, contributing to a better understanding of the hindgut system as a whole.