Human activity produces ambient noise that potentially alters species’ abilities to communicate with each other—among other impacts. Given that birds are known to be sensitive to structural changes in habitat and highly communicative through sound, it is beneficial to understand how changing acoustic ecologies and ambient noise impact birds’ ability to communicate in their respective environments. In this study, mockingbird calls from an urban, desert, and intermediate study site were recorded and analyzed for differences in acoustic properties. Acoustic properties such as frequency and amplitude differed significantly across sites as it was determined that mockingbirds in urban areas increase both the peak frequency and amplitude of their calls in order to communicate. This study identifies what these changes in acoustic properties mean in relation to the survival and conservation of birds and concludes with recommendations for novel research.