Double-flow Focused Liquid Injector for Efficient Serial Femtosecond Crystallography
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.
- Author (aut): Oberthuer, Dominik
- Author (aut): Knoska, Juraj
- Author (aut): Wiedorn, Max O.
- Author (aut): Beyerlein, Kenneth R.
- Author (aut): Bushnell, David A.
- Author (aut): Kovaleva, Elena G.
- Author (aut): Heymann, Michael
- Author (aut): Gumprecht, Lars
- Author (aut): Kirian, Richard
- Author (aut): Barty, Anton
- Author (aut): Mariani, Valerio
- Author (aut): Tolstikova, Aleksandra
- Author (aut): Adriano, Luigi
- Author (aut): Awel, Salah
- Author (aut): Barthelmess, Miriam
- Author (aut): Dorner, Katerina
- Author (aut): Xavier, P. Lourdu
- Author (aut): Yefanov, Oleksandr
- Author (aut): James, Daniel
- Author (aut): Nelson, Garrett
- Author (aut): Wang, Dingjie
- Author (aut): Calvey, George
- Author (aut): Chen, Yujie
- Author (aut): Schmidt, Andrea
- Author (aut): Szczepek, Michael
- Author (aut): Frielingsdorf, Stefan
- Author (aut): Lenz, Oliver
- Author (aut): Snell, Edward
- Author (aut): Robinson, Philip J.
- Author (aut): Sarler, Bozidar
- Author (aut): Belsak, Grega
- Author (aut): Macek, Marjan
- Author (aut): Wilde, Fabian
- Author (aut): Aquila, Andrew
- Author (aut): Boutet, Sebastien
- Author (aut): Liang, Mengning
- Author (aut): Hunter, Mark S.
- Author (aut): Scheerer, Patrick
- Author (aut): Lipscomb, John D.
- Author (aut): Weierstall, Uwe
- Author (aut): Kornberg, Roger D.
- Author (aut): Spence, John
- Author (aut): Pollack, Lois
- Author (aut): Chapman, Henry N.
- Author (aut): Bajt, Sasa
- Contributor (ctb): College of Liberal Arts and Sciences
- Contributor (ctb): Department of Physics