In the realm of environmental engineering, the compound N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD), has recently emerged as an environmental concern. 6PPD serves as a tire additive to prolong the lifespan of rubber but can transform into a more toxic derivative, N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine-quinone (6PPD-quinone), when exposed to ground-level ozone. Initially, my research sought to investigate the biodegradation of 6PPD and 6PPD-quinone using microbial cultures. However, unexpected challenges arising from limited solubility and potential toxicity to microorganisms led to a shift in research objectives. The study then refocused on developing methods for detecting and quantifying 6PPD and 6PPD-quinone. The scarcity of literature available on the environmental fate and transport of these compounds underscores the pressing need for further research to gain a comprehensive understanding of the behavior of these chemicals. Consequently, the development of effective detection strategies will enable the development of effective remediation strategies to safeguard aquatic ecosystems.