The purpose of this experiment was to use real-time quantitative polymerase chain reactions (RT-qPCR) to quantify and analyze differences in expression of U1 snRNA variants across four different human Leukemia cell lines. We found a number of interesting results in the four cell lines. Two variants in particular (vU1.15 and vU1.19), were only expressed in one leukemia cell line each, indicating a potential link between their specific mutations and the type of leukemia associated with the cell lines in which they were expressed. Further research should be conducted to understand these differences and uncover potential clinical applications.