Enzyme Induced Carbonate Precipitation: Examining Protein-Enhanced Adhesion and Application for Surficial Stabilization of a Mine Tailing

The introduction of milk powder into conventional Enzyme Induced Carbonate Precipitation (EICP) solutions, comprised of urea, calcium chloride, and urease enzyme, has been shown to increase the unconfined compressive strength of treated soil columns. The casein protein in milk is well known for its adhesive properties and could potentially alter the soil-crystal interface, enhancing the adhesion. This research primarily seeks to compare the difference in adhesion of EICP precipitates without powdered milk and those modified with powdered milk, while also examining the distribution of milk proteins within the samples. Borosilicate glass slides (22mm × 22mm) served as representative substrates for soil in this investigation. Additionally, the study evaluates the viability of EICP treatment for surficial stabilization of mine tailings, employing a combination of laboratory experiments and large-scale field deployments for feasibility analysis.