Description
This thesis describes several approaches to next generation DNA sequencing via tunneling current method based on a Scanning Tunneling Microscope system. In chapters 5 and 6, preliminary results have shown that DNA bases could be identified by their characteristic tunneling signals. Measurements taken in aqueous buffered solution showed that single base resolution could be achieved with economic setups. In chapter 7, it is illustrated that some ongoing measurements are indicating the sequence readout by making linear scan on a piece of short DNA oligomer. However, to overcome the difficulties of controlling DNA especially ssDNA movement, it is much better to have the tunneling measurement incorporated onto a robust nanopore device to realize sequential reading of the DNA sequence while it is being translocated.
Details
Title
- Recognition tunneling: approaches towards next generation DNA sequencing
Contributors
- Huang, Shuo (Author)
- Lindsay, Stuart (Thesis advisor)
- Sankey, Otto (Committee member)
- Tao, Nongjian (Committee member)
- Drucker, Jeff (Committee member)
- Ros, Robert (Committee member)
- Arizona State University (Publisher)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2011
Subjects
Resource Type
Collections this item is in
Note
- thesisPartial requirement for: Ph.D., Arizona State University, 2011
- bibliographyIncludes bibliographical references (p. 115-125)
- Field of study: Physics
Citation and reuse
Statement of Responsibility
by Shuo Huang