Full metadata
Title
Utilizing Confocal Laser Scanning Microscopy to Visualize Origami Nanostructure Transfection in Primary Immune Cells
Description
An aim of fundamental immunology is quantifying the diversity of the T cell receptor (TCR) repertoire to elucidate the vast recognition by T cells for protection against pathogen and cancer. The utilization of DNA origami nanostructures engineered to capture single cell paired TCR mRNA sequences has transformed the financial and time requirements of repertoire establishment. To further support this protocol, confocal laser scanning microscopy was implemented following transfection to visualize the stability of the DNA origami within primary immune lymphocytes.
Date Created
2018-05
Contributors
- Reed, Abigail Elizabeth (Author)
- Blattman, Joseph (Thesis director)
- Glenn, Honor (Committee member)
- Schoettle, Louis (Committee member)
- School of Life Sciences (Contributor)
- W.P. Carey School of Business (Contributor)
- Barrett, The Honors College (Contributor)
Topical Subject
Resource Type
Extent
24 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Series
Academic Year 2017-2018
Handle
https://hdl.handle.net/2286/R.I.48030
Level of coding
minimal
Cataloging Standards
System Created
- 2018-04-20 12:12:07
System Modified
- 2021-08-11 04:09:57
- 3 years 3 months ago
Additional Formats