Description
An aim of fundamental immunology is quantifying the diversity of the T cell receptor (TCR) repertoire to elucidate the vast recognition by T cells for protection against pathogen and cancer. The utilization of DNA origami nanostructures engineered to capture single cell paired TCR mRNA sequences has transformed the financial and time requirements of repertoire establishment. To further support this protocol, confocal laser scanning microscopy was implemented following transfection to visualize the stability of the DNA origami within primary immune lymphocytes.
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Details
Title
- Utilizing Confocal Laser Scanning Microscopy to Visualize Origami Nanostructure Transfection in Primary Immune Cells
Contributors
- Reed, Abigail Elizabeth (Author)
- Blattman, Joseph (Thesis director)
- Glenn, Honor (Committee member)
- Schoettle, Louis (Committee member)
- School of Life Sciences (Contributor)
- W.P. Carey School of Business (Contributor)
- Barrett, The Honors College (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2018-05
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