Full metadata
Title
Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Description
Zoonotic pathogens that cause leprosy (Mycobacterium leprae) and tuberculosis (Mycobacterium tuberculosis complex, MTBC) continue to impact modern human populations. Therefore, methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. Here we tested for mycobacterial-specific single- and multi-copy loci using qPCR. In a trial study in which armadillos were artificially infected with M. leprae, these techniques were specific and sensitive to pathogen detection, while more traditional ELISAs were only specific. These assays were then employed in a case study to detect M. leprae as well as MTBC in wild marmosets. All marmosets were negative for M. leprae DNA, but 14 were positive for the mycobacterial rpoB gene assay. Targeted capture and sequencing of rpoB and other MTBC genes validated the presence of mycobacterial DNA in these samples and revealed that qPCR is useful for identifying mycobacterial-infected animal hosts.
Date Created
2015-11-16
Contributors
- Housman, Genevieve (Author)
- Malukiewicz, Joanna (Author)
- Boere, Vanner (Author)
- Grativol, Adriana D. (Author)
- Pereira, Luiz Cezar M. (Author)
- de Oliveira e Silva, Ita (Author)
- Ruiz-Miranda, Carlos R. (Author)
- Truman, Richard (Author)
- Stone, Anne (Author)
- College of Liberal Arts and Sciences (Contributor)
- School of Human Evolution and Social Change (Contributor)
- Biodesign Institute (Contributor)
- Personalized Diagnostics (Contributor)
Extent
13 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Identifier
Digital object identifier: 10.1371/journal.pntd.0004198
Identifier Type
International standard serial number
Identifier Value
1935-2727
Identifier Type
International standard serial number
Identifier Value
1935-2735
Series
PLOS NEGLECTED TROPICAL DISEASES
Handle
https://hdl.handle.net/2286/R.I.43781
Preferred Citation
Housman, G., Malukiewicz, J., Boere, V., Grativol, A. D., Pereira, L. C., Silva, I. D., . . . Stone, A. C. (2015). Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs. PLOS Neglected Tropical Diseases, 9(11). doi:10.1371/journal.pntd.0004198
Level of coding
minimal
Cataloging Standards
Note
The article is published at http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004198
System Created
- 2017-05-22 03:45:13
System Modified
- 2021-08-16 02:23:30
- 3 years 3 months ago
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