Description
Zoonotic pathogens that cause leprosy (Mycobacterium leprae) and tuberculosis (Mycobacterium tuberculosis complex, MTBC) continue to impact modern human populations. Therefore, methods able to survey mycobacterial infection in potential animal hosts are necessary for proper evaluation of human exposure threats. Here we tested for mycobacterial-specific single- and multi-copy loci using qPCR. In a trial study in which armadillos were artificially infected with M. leprae, these techniques were specific and sensitive to pathogen detection, while more traditional ELISAs were only specific. These assays were then employed in a case study to detect M. leprae as well as MTBC in wild marmosets. All marmosets were negative for M. leprae DNA, but 14 were positive for the mycobacterial rpoB gene assay. Targeted capture and sequencing of rpoB and other MTBC genes validated the presence of mycobacterial DNA in these samples and revealed that qPCR is useful for identifying mycobacterial-infected animal hosts.
Included in this item (2)
Permanent Link
Permanent Link
Details
Title
- Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs
Contributors
Agent
- Housman, Genevieve (Author)
- Malukiewicz, Joanna (Author)
- Boere, Vanner (Author)
- Grativol, Adriana D. (Author)
- Pereira, Luiz Cezar M. (Author)
- de Oliveira e Silva, Ita (Author)
- Ruiz-Miranda, Carlos R. (Author)
- Truman, Richard (Author)
- Stone, Anne (Author)
- College of Liberal Arts and Sciences (Contributor)
- School of Human Evolution and Social Change (Contributor)
- Biodesign Institute (Contributor)
- Personalized Diagnostics (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2015-11-16
Collections this item is in
Identifier
- Digital object identifier: 10.1371/journal.pntd.0004198
- Identifier TypeInternational standard serial numberIdentifier Value1935-2727
- Identifier TypeInternational standard serial numberIdentifier Value1935-2735
Note
- The article is published at http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0004198
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Housman, G., Malukiewicz, J., Boere, V., Grativol, A. D., Pereira, L. C., Silva, I. D., . . . Stone, A. C. (2015). Validation of qPCR Methods for the Detection of Mycobacterium in New World Animal Reservoirs. PLOS Neglected Tropical Diseases, 9(11). doi:10.1371/journal.pntd.0004198