Description
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65–70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. The data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
Details
Title
- Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
Contributors
- Reddy, Hemanth K. N. (Author)
- Yoon, Chun Hong (Author)
- Aquila, Andrew (Author)
- Awel, Salah (Author)
- Ayyer, Kartik (Author)
- Barty, Anton (Author)
- Berntsen, Peter (Author)
- Bielecki, Johan (Author)
- Bobkov, Sergey (Author)
- Bucher, Maximilian (Author)
- Carini, Gabriella A. (Author)
- Carron, Sebastian (Author)
- Chapman, Henry (Author)
- Daurer, Benedikt (Author)
- DeMirci, Hasan (Author)
- Ekeberg, Tomas (Author)
- Fromme, Petra (Author)
- Hajdu, Janos (Author)
- Hanke, Max Felix (Author)
- Hart, Philip (Author)
- Hogue, Brenda (Author)
- Hasseinizadeh, Ahmad (Author)
- Kim, Yoonhee (Author)
- Kirian, Richard (Author)
- Kurta, Ruslan P. (Author)
- Larsson, Daniel S. D. (Author)
- Loh, N. Duane (Author)
- Maia, Filipe R. N. C. (Author)
- Mancuso, Adrian P. (Author)
- Muhlig, Kerstin (Author)
- Munke, Anna (Author)
- Nam, Daewoong (Author)
- Nettelblad, Carl (Author)
- Ourmazd, Abbas (Author)
- Rose, Max (Author)
- Schwander, Peter (Author)
- Seibert, Marvin (Author)
- Sellberg, Jonas A. (Author)
- Song, Changyong (Author)
- Spence, John (Author)
- Svenda, Martin (Author)
- van der Schot, Gijs (Author)
- Vartanyants, Ivan A. (Author)
- Williams, Garth J. (Author)
- Xavier, P. Lourdu (Author)
- ASU Biodesign Center Immunotherapy, Vaccines and Virotherapy (Contributor)
- Biodesign Institute (Contributor)
- Applied Structural Discovery (Contributor)
- College of Liberal Arts and Sciences (Contributor)
- School of Molecular Sciences (Contributor)
- School of Life Sciences (Contributor)
- Department of Physics (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2017-06-27
Resource Type
Collections this item is in
Identifier
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Digital object identifier: 10.1038/sdata.2017.79
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Identifier TypeInternational standard serial numberIdentifier Value2052-4463
Note
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The final version of this article, as published in Scientific Data, can be viewed online at: http://www.nature.com/articles/sdata201779
Citation and reuse
Cite this item
This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.
Reddy, H. K., Yoon, C. H., Aquila, A., Awel, S., Ayyer, K., Barty, A., . . . Xavier, P. L. (2017). Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source. Scientific Data, 4, 170079. doi:10.1038/sdata.2017.79