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Sera from patients with ovarian cancer contain autoantibodies (AAb) to tumor-derived proteins that are potential biomarkers for early detection. To detect AAb, we probed high-density programmable protein microarrays (NAPPA) expressing 5177 candidate tumor antigens with sera from patients with serous ovarian cancer (n = 34 cases/30 controls) and measured bound IgG. Of these, 741 antigens were selected and probed with an independent set of ovarian cancer sera (n = 60 cases/60 controls). Twelve potential autoantigens were identified with sensitivities ranging from 13 to 22% at >93% specificity. These were retested using a Luminex bead array using 60 cases and 60 controls, with sensitivities ranging from 0 to 31.7% at 95% specificity. Three AAb (p53, PTPRA, and PTGFR) had area under the curve (AUC) levels >60% (p < 0.01), with the partial AUC (SPAUC) over 5 times greater than for a nondiscriminating test (p < 0.01). Using a panel of the top three AAb (p53, PTPRA, and PTGFR), if at least two AAb were positive, then the sensitivity was 23.3% at 98.3% specificity. AAb to at least one of these top three antigens were also detected in 7/20 sera (35%) of patients with low CA 125 levels and 0/15 controls. AAb to p53, PTPRA, and PTGFR are potential biomarkers for the early detection of ovarian cancer.
- Anderson, Karen (Author)
- Cramer, Daniel W. (Author)
- Sibani, Sahar (Author)
- Wallstrom, Garrick (Author)
- Wong, Jessica (Author)
- Park, Jin (Author)
- Qiu, Ji (Author)
- Vitonis, Allison (Author)
- LaBaer, Joshua (Author)
- Biodesign Institute (Contributor)
Anderson, Karen S., Cramer, Daniel W., Sibani, Sahar, Wallstrom, Garrick, Wong, Jessica, Park, Jin, Qiu, Ji, Vitonis, Allison, & LaBaer, Joshua (2015). Autoantibody Signature for the Serologic Detection of Ovarian Cancer. JOURNAL OF PROTEOME RESEARCH, 14(1), 578-586. http://dx.doi.org/10.1021/pr500908n
JOURNAL OF PROTEOME RESEARCH, copyright American Chemical Society, after peer review and technical editing by the publisher.
To access the final published work, see http://dx.doi.org/10.1021/pr500908n
- 2015-04-29 12:19:52
- 2021-10-25 05:16:27
- 3 years ago