Description

Sera from patients with ovarian cancer contain autoantibodies (AAb) to tumor-derived proteins that are potential biomarkers for early detection. To detect AAb, we probed high-density programmable protein microarrays (NAPPA) expressing 5177 candidate tumor antigens with sera from patients with serous

Sera from patients with ovarian cancer contain autoantibodies (AAb) to tumor-derived proteins that are potential biomarkers for early detection. To detect AAb, we probed high-density programmable protein microarrays (NAPPA) expressing 5177 candidate tumor antigens with sera from patients with serous ovarian cancer (n = 34 cases/30 controls) and measured bound IgG. Of these, 741 antigens were selected and probed with an independent set of ovarian cancer sera (n = 60 cases/60 controls). Twelve potential autoantigens were identified with sensitivities ranging from 13 to 22% at >93% specificity. These were retested using a Luminex bead array using 60 cases and 60 controls, with sensitivities ranging from 0 to 31.7% at 95% specificity. Three AAb (p53, PTPRA, and PTGFR) had area under the curve (AUC) levels >60% (p < 0.01), with the partial AUC (SPAUC) over 5 times greater than for a nondiscriminating test (p < 0.01). Using a panel of the top three AAb (p53, PTPRA, and PTGFR), if at least two AAb were positive, then the sensitivity was 23.3% at 98.3% specificity. AAb to at least one of these top three antigens were also detected in 7/20 sera (35%) of patients with low CA 125 levels and 0/15 controls. AAb to p53, PTPRA, and PTGFR are potential biomarkers for the early detection of ovarian cancer.

Downloads
PDF (545 KB)

Details

Title
  • Autoantibody Signature for the Serologic Detection of Ovarian Cancer
Contributors
Date Created
2015-01-01
Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1021/pr500908n
    • Identifier Type
      International standard serial number
      Identifier Value
      1535-3893
    • Identifier Type
      International standard serial number
      Identifier Value
      1535-3907
    Note
    • This document is the Accepted Manuscript version of a Published Work that appeared in final form in
      JOURNAL OF PROTEOME RESEARCH, copyright American Chemical Society, after peer review and technical editing by the publisher.
      To access the final published work, see http://dx.doi.org/10.1021/pr500908n

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Anderson, Karen S., Cramer, Daniel W., Sibani, Sahar, Wallstrom, Garrick, Wong, Jessica, Park, Jin, Qiu, Ji, Vitonis, Allison, & LaBaer, Joshua (2015). Autoantibody Signature for the Serologic Detection of Ovarian Cancer. JOURNAL OF PROTEOME RESEARCH, 14(1), 578-586. http://dx.doi.org/10.1021/pr500908n

    Machine-readable links