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In vitro models that mimic in vivo host-pathogen interactions are needed to evaluate candidate drugs that inhibit bacterial virulence traits. We established a new approach to study Pseudomonas aeruginosa biofilm susceptibility on biotic surfaces, using a three-dimensional (3-D) lung epithelial

In vitro models that mimic in vivo host-pathogen interactions are needed to evaluate candidate drugs that inhibit bacterial virulence traits. We established a new approach to study Pseudomonas aeruginosa biofilm susceptibility on biotic surfaces, using a three-dimensional (3-D) lung epithelial cell model. P. aeruginosa formed antibiotic resistant biofilms on 3-D cells without affecting cell viability. The biofilm-inhibitory activity of antibiotics and/or the anti-biofilm peptide DJK-5 were evaluated on 3-D cells compared to a plastic surface, in medium with and without fetal bovine serum (FBS). In both media, aminoglycosides were more efficacious in the 3-D cell model. In serum-free medium, most antibiotics (except polymyxins) showed enhanced efficacy when 3-D cells were present. In medium with FBS, colistin was less efficacious in the 3-D cell model. DJK-5 exerted potent inhibition of P. aeruginosa association with both substrates, only in serum-free medium. DJK-5 showed stronger inhibitory activity against P. aeruginosa associated with plastic compared to 3-D cells. The combined addition of tobramycin and DJK-5 exhibited more potent ability to inhibit P. aeruginosa association with both substrates. In conclusion, lung epithelial cells influence the efficacy of most antimicrobials against P. aeruginosa biofilm formation, which in turn depends on the presence or absence of FBS.

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    Title
    • Antimicrobial Efficacy Against Pseudomonas Aeruginosa Biofilm Formation in a Three-Dimensional Lung Epithelial Model and the Influence of Fetal Bovine Serum
    Date Created
    2017-03-03
    Resource Type
  • Text
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    Identifier
    • Digital object identifier: 10.1038/srep43321
    • Identifier Type
      International standard serial number
      Identifier Value
      2045-2322
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    • The final version of this article, as published in Scientific Reports, can be viewed online at: http://www.nature.com/articles/srep43321

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    Crabbé, A., Liu, Y., Matthijs, N., Rigole, P., Fuente-Nùñez, C. D., Davis, R., . . . Nickerson, C. A. (2017). Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum. Scientific Reports, 7, 43321. doi:10.1038/srep43321

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