Description

Recent epigenetic association studies have identified a new gene, ANK1, in the pathogenesis of Alzheimer’s disease (AD). Although strong associations were observed, brain homogenates were used to generate the data, introducing complications because of the range of cell types analyzed.

Recent epigenetic association studies have identified a new gene, ANK1, in the pathogenesis of Alzheimer’s disease (AD). Although strong associations were observed, brain homogenates were used to generate the data, introducing complications because of the range of cell types analyzed. In order to address the issue of cellular heterogeneity in homogenate samples we isolated microglial, astrocytes and neurons by laser capture microdissection from CA1 of hippocampus in the same individuals with a clinical and pathological diagnosis of AD and matched control cases. Using this unique RNAseq data set, we show that in the hippocampus, ANK1 is significantly (p<0.0001) up-regulated 4-fold in AD microglia, but not in neurons or astrocytes from the same individuals. These data provide evidence that microglia are the source of ANK1 differential expression previously identified in homogenate samples in AD.

Downloads
PDF (1.5 MB)

Details

Title
  • ANK1 is Up-Regulated in Laser Captured Microglia in Alzheimer’s Brain: The Importance of Addressing Cellular Heterogeneity
Contributors
Date Created
2017-07-12
Resource Type
  • Text
  • Collections this item is in
    Identifier
    • Digital object identifier: 10.1371/journal.pone.0177814
    • Identifier Type
      International standard serial number
      Identifier Value
      1045-3830
    • Identifier Type
      International standard serial number
      Identifier Value
      1939-1560
    Note
    • The article is published at http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0177814

    Citation and reuse

    Cite this item

    This is a suggested citation. Consult the appropriate style guide for specific citation guidelines.

    Mastroeni, D., Sekar, S., Nolz, J., Delvaux, E., Lunnon, K., Mill, J., . . . Coleman, P. D. (2017). ANK1 is up-regulated in laser captured microglia in Alzheimer’s brain; the importance of addressing cellular heterogeneity. Plos One, 12(7). doi:10.1371/journal.pone.0177814

    Machine-readable links