Adverse neighborhood environments have been found to negatively influence children’s sleep and physical activity outcomes (Pabayo et al., 2014; Aguilar-Farias et al., 2020). Previous literature suggests that positive parenting and familism values may play protective roles in high-risk neighborhood contexts (Romero et al., 2020). This study utilized a strengths-based approach to examine relations between neighborhood opportunities and sleep and physical activity in children, and whether positive parenting and familism values buffered these associations. This study utilized a racially and socioeconomically diverse sample of 710 twin children (Mage=8.44 years, SD=.69; 51.4% female; 58.5% non-Hispanic White, 23.7% Hispanic). Children wore actigraphy watches for 7 nights (M=6.79, SD=.71) to capture sleep (duration, efficiency, midpoint, midpoint variability) and physical activity (MVPA and sedentary behaviors) parameters. Objective neighborhood opportunity was evaluated using the Child Opportunity Index 2.0 (COI; Noelke et al., 2020). Positive parenting behaviors were observed and coded in the context of a parent-child interaction task. Primary caregivers reported on their children’s sleep problems and their familism values. Results revealed that higher COI predicted earlier sleep midpoint and less midpoint variability. Interactions between COI and positive parenting were nonsignificant. Though marginal, associations between the COI and midpoint variability were most negatively related in children whose primary caregiver reported high familism values. Future research should look at children’s engagement in family activities to better understand whether children who reside in low opportunity neighborhoods and experience high familism values have to face additional competing family demands, leading to greater individual variation in sleep timing.