Description
Adoptive cell therapies such as chimeric antigen receptor (CAR) modified immune cells are revolutionizing cancer treatment. These innovative immunotherapies have a promising outlook for liquid cancers, but lack robustness against solid tumors due to complex variables introduced by the tumor microenvironment (TME). Additionally, existing CAR-T cell treatments are commonly accompanied by toxic side effects. However, by grafting a CAR construct onto macrophages, a professional phagocytic innate cell which are actively recruited by solid tumors, the efficacy of this treatment is hoped to be extended beyond hematological malignancies. Moreover, the introduction of energy metabolite-based polymers (EMPs) to provide a sustained release of activating F16BP-poly(I:C) microparticles could address the toxicity complications that arise from CAR treatments. It was determined that PLGA-F16BP-poly(I:C) microparticles allow for CAR-macrophage activation in vitro, though not in a sustained manner. Moreover, F16BP-poly(I:C) microparticles were better geared toward reducing cytokine related toxicity in vitro, with in vivo results remaining inconclusive. These findings suggest prioritization between macrophage activation or cytokine storm reduction would be required at this time, though additional future studies to explore variables such as CAR-macrophage sensitivity and the positive control could help refine this immunotherapy.
Details
Title
- Investigating the Potential of F16BP-poly(I:C) Microparticle Associated CAR Therapies
Contributors
- Halim, Michelle (Author)
- Acharya, Abhinav P (Thesis advisor)
- Holloway, Julianne (Committee member)
- Khalifehzadeh, Layla (Committee member)
- Arizona State University (Publisher)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2023
Resource Type
Collections this item is in
Note
- Partial requirement for: M.S., Arizona State University, 2023
- Field of study: Chemical Engineering