Description
The purpose of this experiment is to deliver DNA origami barrels loaded with Cas13d-gRNA binary complexes to HPV-16 and HPV-18 cervical cancer lines to make the cancer mortal. The production of Cas 13d has proven successful with a concentration of ~ 1mg/mL, but the activity assay performed has not shown conclusive evidence of Cas13d and guide RNA binary complex formation or activity. Successful annealing of the DNA origami barrel has been quantified by an agarose gel, but further quantification by TEM is in progress. Overall, steady progress is being made towards the goal of targeting HPV16 E6/E7 pre-mRNA with CRISPR/Cas13d.
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Title
- CRISPR Cas13d Conjugated to a DNA ORIGAMI Barrel to inactivate cervical cancer caused by HPV.
Contributors
- Gamoth, Yash (Author)
- Anderson, Karen (Thesis director)
- Chu, Po Lin (Committee member)
- Lapinaite, Audrone (Committee member)
- Barrett, The Honors College (Contributor)
- School of Molecular Sciences (Contributor)
- Dean, W.P. Carey School of Business (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2023-05
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