Secondary Analysis on the Impact of Sleep-Wake Patterns and Feeding Practices in the Gut Microbiome in the First Years of Life Trailed into Toddlerhood

Background:The second an individual is born, the gut microbiome starts acquiring unique characteristics, including microbial richness and evenness. In addition, it has been found that during infancy, the mode of delivery, antibiotic exposure, feeding patterns, and environment play a role in such development. However, infancy is still an understudied population related to the gut microbiota, specifically its’ connection to two modifiable factors-sleep-wake patterns and its’ interconnection with feeding practices in the first year of life. Methods: This secondary data analysis from a randomized longitudinal intervention study assessed the efficacy of a home-based education program in preventing the onset of childhood obesity. A convenience sample of 40 Hispanic mother and infant dyads were recruited to participate in an additional collection of fecal samples to evaluate associations between lifestyle/behavioral factors in infancy and gut microbiome composition in toddlerhood. Total sleep duration and feeding practices (breastmilk and formula) were assessed at one, six, and twelve months. In addition, alpha and beta diversity metrics were assessed from infant stool samples collected at 36 months. Results: This study found some significant and trending values for pairwise comparisons of alpha (Shannon Diversity Index) and beta (Bray Curtis and Jaccard Distance). Sleep-wake adequacy consisted of 14-17 total hours of sleep in 24 hours at one month and 12-16 hours for six and twelve months of age. No significant values were identified at one month of age. However, six and twelve months demonstrated significant observations for gut microbial richness and evenness. Trending differences (p=.06, Shannon Diversity Index) persisted in infants receiving adequate sleep at six months but for different feeding modalities. Faith’s PD, Pielou’s measure, and observed OTUs were three additional alpha-diversity metrics performed in all groups. Observed OTUs (p=.03) and Faith’s PD (p=.03) were significant at twelve months, demonstrating an increased microbial feature in infants receiving adequate sleep. Conclusion: The findings from this study may show how different timestamps in the first year of life may create gut microbial milestones. However, the interrelation between sleep-wake patterns, feeding modalities, and gut microbiome development is limited; further investigation is needed to monitor close changes and potentially create a criterion