Full metadata
Title
Targeted Metabolomics Reveals the Effect of Nitrate Supplementation on Vascular Function
Description
In the United States, two-thirds of adults are considered hypertensive orprehypertensive. In addition, chronic illness, such as hypertension, cardiovascular disease, and type II diabetes, results in $3.5 trillion in annual healthcare cost and is the primary cause of disability and death. As a result, many individuals seek cheaper and simpler alternatives to combat their conditions. In this exploratory analysis, a study assessing nitrate intake and its effects on vascular function in 39 young adult males was investigated for underlying metabolic variations through a liquid chromatography – mass spectrometry-based large-scale targeted metabolomics approach. A two-way repeated measures ANOVA was used, and 18 significant metabolites were discovered across the time, treatment, and time & treatment groups, including prostaglandin E2 (p<0.001), stearic acid (p=0.002), caprylic acid (p=0.016), pentadecanoic acid (p=0.027), and heptadecanoic acid (p=0.005). In addition, log-transformed principal component analysis and orthogonal partial least squares – discriminant analysis models demonstrated distinct separation among the treatment, control, and time variables. Moreover, pathway and enrichment analyses validated the effect of nitrate intake on the metabolite sets and its possible function in fatty acid oxidation. This better understanding of altered metabolic pathways may help explicate the benefits of nitrate on vascular function and reveal any unknown mechanisms of its supplementation.
Date Created
2020
Contributors
- Patterson, Jeffrey (Author)
- Gu, Haiwei (Thesis advisor)
- Johnston, Carol (Committee member)
- Sweazea, Karen (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
92 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.62984
Level of coding
minimal
Note
Masters Thesis Nutrition 2020
System Created
- 2021-01-14 09:17:18
System Modified
- 2021-08-26 09:47:01
- 3 years 2 months ago
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