Description
In this thesis, a breadboard Integrated Microarray Printing and Detection System (IMPDS) was proposed to address key limitations of traditional microarrays. IMPDS integrated two core components of a high-resolution surface plasmon resonance imaging (SPRi) system and a piezoelectric dispensing system that can print ultra-low volume droplets. To avoid evaporation of droplets in the microarray, a 100 μm thick oil layer (dodecane) was used to cover the chip surface. The interaction between BSA (Bovine serum albumin) and Anti-BSA was used to evaluate the capability of IMPDS. The alignment variability of printing, stability of droplets array and quantification of protein-protein interactions based on nanodroplet array were evaluated through a 10 x 10 microarray on SPR sensor chip. Binding kinetic constants obtained from IMPDS are close with results from commercial SPR setup (BI-3000), which indicates that IMPDS is capable to measure kinetic constants accurately. The IMPDS setup has following advantages: 1) nanoliter scale sample consumption, 2) high-throughput detection with real-time kinetic information for biomolecular interactions, 3) real-time information during printing and spot-on-spot detection of biomolecular interactions 4) flexible selection of probes and receptors (M x N interactions). Since IMPDS studies biomolecular interactions with low cost and high flexibility in real-time manner, it has great potential in applications such as drug discovery, food safety and disease diagnostics, etc.
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Details
Title
- An integrated microarray printing and detection system to study protein-protein interactions
Contributors
- Xiao, Feng (Author)
- Tao, Nongjian (Thesis advisor)
- Borges, Chad (Committee member)
- Guo, Jia (Committee member)
- Arizona State University (Publisher)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2017
Subjects
Resource Type
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Note
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thesisPartial requirement for: M.S., Arizona State University, 2017
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bibliographyIncludes bibliographical references (pages 31-33)
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Field of study: Chemistry
Citation and reuse
Statement of Responsibility
by Feng Xiao