Description
ABSTRACT In terms of prevalence, human suffering and costs dengue infections are the most important arthropod-borne viral disease worldwide. Dengue virus (DENV) is a mosquito-borne flavivirus and the etiological agent of dengue fever and dengue hemorrhagic fever. Thus, development of a safe and efficient vaccine constitutes an urgent necessity. Besides the traditional strategies aim at generating immunization options, the usage of viral vectors to deliver antigenic stimulus in order to elicit protection are particularly attractive for the endeavor of a dengue vaccine. The viral vector (MVvac2) is genetically equivalent to the currently used measles vaccine strain Moraten, which adds practicality to my approach. The goal of the present study was to generate a recombinant measles virus expressing structural antigens from two strains of DENV (DENV2 and DENV4) The recombinant vectors replication profile was comparable to that of the parental strain and expresses either membrane bound or soluble forms of DENV2 and DENV4 E glycoproteins. I discuss future experiments in order to demonstrate its immunogenicity in our measles-susceptible mouse model.
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Title
- Expression of dengue virus envelope glycoproteins using a measles vaccine vector
Contributors
- Abdelgalel, Rowida (Author)
- Reyes del Valle, Jorge (Thesis advisor)
- Hogue, Brenda (Committee member)
- Frasch, Wayne D (Committee member)
- Arizona State University (Publisher)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2013
Subjects
Resource Type
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Note
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thesisPartial requirement for: M.S., Arizona State University, 2013
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bibliographyIncludes bibliographical references (p. 99-109)
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Field of study: Biology
Citation and reuse
Statement of Responsibility
by Rowida Abdelgalel