Description
Single molecule DNA Sequencing technology has been a hot research topic in the recent decades because it holds the promise to sequence a human genome in a fast and affordable way, which will eventually make personalized medicine possible. Single molecule differentiation and DNA translocation control are the two main challenges in all single molecule DNA sequencing methods. In this thesis, I will first introduce DNA sequencing technology development and its application, and then explain the performance and limitation of prior art in detail. Following that, I will show a single molecule DNA base differentiation result obtained in recognition tunneling experiments. Furthermore, I will explain the assembly of a nanofluidic platform for single strand DNA translocation, which holds the promised to be integrated into a single molecule DNA sequencing instrument for DNA translocation control. Taken together, my dissertation research demonstrated the potential of using recognition tunneling techniques to serve as a general readout system for single molecule DNA sequencing application.
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Details
Title
- Towards single molecule DNA sequencing
Contributors
- Liu, Hao (Author)
- Lindsay, Stuart M (Committee member)
- Yan, Hao (Committee member)
- Levitus, Marcia (Committee member)
- Arizona State University (Publisher)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2013
Subjects
Resource Type
Collections this item is in
Note
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thesisPartial requirement for: Ph.D., Arizona State University, 2013
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bibliographyIncludes bibliographical references (p. 66-75)
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Field of study: Biochemistry
Citation and reuse
Statement of Responsibility
by Hao Liu