Full metadata
Title
Replication of DNA tetrahedron and higher-order self-assembly of DNA origami
Description
Deoxyribonucleic acid (DNA) has been treated as excellent building material for nanoscale construction because of its unique structural features. Its ability to self-assemble into predictable and addressable nanostructures distinguishes it from other materials. A large variety of DNA nanostructures have been constructed, providing scaffolds with nanometer precision to organize functional molecules. This dissertation focuses on developing biologically replicating DNA nanostructures to explore their biocompatibility for potential functions in cells, as well as studying the molecular behaviors of DNA origami tiles in higher-order self-assembly for constructing DNA nanostructures with large size and complexity. Presented here are a series of studies towards this goal. First, a single-stranded DNA tetrahedron was constructed and replicated in vivo with high efficiency and fidelity. This study indicated the compatibility between DNA nanostructures and biological systems, and suggested a feasible low-coast method to scale up the preparation of synthetic DNA. Next, the higher-order self-assembly of DNA origami tiles was systematically studied. It was demonstrated that the dimensional aspect ratio of origami tiles as well as the intertile connection design were essential in determining the assembled superstructures. Finally, the effects of DNA hairpin loops on the conformations of origami tiles as well as the higher-order assembled structures were demonstrated. The results would benefit the design and construction of large complex nanostructures.
Date Created
2012
Contributors
- Li, Zhe (Author)
- Yan, Hao (Thesis advisor)
- Liu, Yan (Thesis advisor)
- Seo, Dong-Kyun (Committee member)
- Wachter, Rebekka (Committee member)
- Arizona State University (Publisher)
Topical Subject
Resource Type
Extent
viii, 201 p. : ill. (some col.)
Language
eng
Copyright Statement
In Copyright
Primary Member of
Peer-reviewed
No
Open Access
No
Handle
https://hdl.handle.net/2286/R.I.14592
Statement of Responsibility
by Zhe Li
Description Source
Viewed on Feb. 19, 2013
Level of coding
full
Note
thesis
Partial requirement for: Ph.D., Arizona State University, 2012
bibliography
Includes bibliographical references
Field of study: Biochemistry
System Created
- 2012-08-24 06:16:43
System Modified
- 2021-08-30 01:48:26
- 3 years 2 months ago
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