Description
A coincidence reporter construct, consisting of the p21-promoter and two luciferase genes (Firefly and Renilla), was constructed for the screening of drugs that might inhibit Olig2's tumorigenic role in glioblastoma. The reporter construct was tested using an Olig2 inhibitor, HSP990, as well as short hairpin RNA targeting Olig2. Further confirmatory analysis is needed before the reporter cell line is ready for high-throughput screening at the NIH and lead compound selection.
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Title
- Glioblastoma in the Crosshairs: Development of a Dual Reporter Assay for Discovery of Olig2 Inhibiting Drugs
Contributors
- Cusimano, Joseph Michael (Author)
- LaBaer, Joshua (Thesis director)
- Mangone, Marco (Committee member)
- Mehta, Shwetal (Committee member)
- Barrett, The Honors College (Contributor)
- Department of Chemistry and Biochemistry (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2014-05
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