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Title
Characterization of a Polyclonal Antibody Specific for Synechococcus WH8102 Plastoquinol Terminal Oxidase.
Description
Photosynthesis is a critical process that fixes the carbon utilized in cellular respiration. In higher plants, the immutans gene codes for a protein that is both involved in carotenoid biosynthesis and plastoquinol oxidation (Carol et al 1999, Josse et al 2003). This plastoquinol terminal oxidase (PTOX) is of great interest in understanding electron flow in the plastoquinol pool. In order to characterize this PTOX, polyclonal antibodies were developed. Expression of Synechococcus WH8102 PTOX in E. coli provided a useful means to harvest the protein required for antibody production. Once developed, the antibody was tested for limit of concentration, effectiveness in whole cell lysate, and overall specificity. The antibody raised against PTOX was able to detect as low as 10 pg of PTOX in SDS-PAGE, and could detect PTOX extracted from lysed Synechococcus WH8102. The production of this antibody could determine the localization of the PTOX in Synechococcus.
Date Created
2014-05
Contributors
- Khan, Mohammad Iqbal (Author)
- Moore, Thomas (Thesis director)
- Redding, Kevin (Committee member)
- Roberson, Robert (Committee member)
- Barrett, The Honors College (Contributor)
- Department of Chemistry and Biochemistry (Contributor)
- School of Life Sciences (Contributor)
Topical Subject
Resource Type
Extent
12 pages
Language
eng
Copyright Statement
In Copyright
Primary Member of
Series
Academic Year 2013-2014
Handle
https://hdl.handle.net/2286/R.I.23338
Level of coding
minimal
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System Created
- 2017-10-30 02:50:57
System Modified
- 2021-08-11 04:09:57
- 3 years 3 months ago
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