Description
Microwave hydrolysis of egg-white lysozyme was optimized using 1H liquid-state nuclear magnetic resonance (NMR) spectroscopy experiments for amino acid analysis (AAA). Time held under microwave hydrolysis was arrayed for 2, 4, 6, 8, 10, and 15 minutes. Correlations from gCOSY 2D NMR experiments combined with 1H assignments in the one-dimensional chemical shift spectra identified 18 of the 20 amino acids found in lysozyme. Comparison with Uniprot database amino acid composition values revealed the optimal microwave hydrolysis time lies between 4 and 6 minutes. Identification of lysozyme was confirmed with the ExPASy online database search tool AACompIdent. The microwave hydrolysis procedure presented is a simple analytical technique allowing quick and reliable sample preparation in less than one hour that requires no separation or derivation of amino acids residues prior to detection.
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Details
Title
- Optimizing Microwave Hydrolysis for 1H NMR Amino Acid Analysis of Protein Biopolymers
Contributors
Agent
- Edwards, Maximillian Ashur (Author)
- Yarger, Jeff (Thesis director)
- Marzke, Robert (Committee member)
- Barrett, The Honors College (Contributor)
- School of Mathematical and Statistical Sciences (Contributor)
- Department of Chemistry and Biochemistry (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2015-05
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