Description
Circular Dichroism (CD) and electron paramagnetic resonance (EPR) were used to investigate the metal-binding sites of five different four-helix bundles, which have slight differences in the population of their side chains. Of the four-helix bundles, three have central dinuclear metal binding sites; two of these three also have outer dinuclear metal binding sites. The other two peptides have two identical, non-central, dinuclear metal binding sites. The CD spectra showed changes in the secondary structure of the peptides, and X-band EPR spectra of these peptides revealed the unique four peak signal of Cu(II). These findings improve our understanding of the metal binding environments of these peptides.
Details
Title
- Investigation of Cu(II) Binding Sites in de Novo Proteins by Electron Paramagnetic Resonance Spectroscopy
Contributors
- Canarie, Elizabeth Rose (Author)
- Allen, James (Thesis director)
- Wolf, George (Committee member)
- School of Mathematical and Statistical Sciences (Contributor)
- School of Molecular Sciences (Contributor)
- Barrett, The Honors College (Contributor)
Date Created
The date the item was original created (prior to any relationship with the ASU Digital Repositories.)
2016-05
Resource Type
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