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Description
Atomic force microscopy (AFM) was used to study structural differences in the chromatin of cancerous (CP-D) and non-cancerous (EPC2) cell lines. Chromatin samples were extracted using a salt fractionation protocol and subject to Mnase digestion for 2, 4, 8,

Atomic force microscopy (AFM) was used to study structural differences in the chromatin of cancerous (CP-D) and non-cancerous (EPC2) cell lines. Chromatin samples were extracted using a salt fractionation protocol and subject to Mnase digestion for 2, 4, 8, and 16 minutes. Samples were then immobilized on APTES-functionalized mica sheets. Images were produced using the tapping mode capabilities of the AFM and structural differences between cell lines were quantified using image processing software. Vast differences in chromatin structure were observed between cancerous and non-cancerous cell lines and it was discovered that CP-D chromatin is present as scattered nucleosomes and nucleosome aggregates while EPC2 chromatin is present in intricate arrays. It was also observed that in both the CP-D and EPC2 cell lines, nucleosomes were more isolated and less apparent at longer Mnase digestion times. These findings lead to the conclusion that as the DNA becomes sufficiently digested, chromatin and nucleosomal arrays begin to deteriorate and lose their complex and elaborate structure.


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Details

Title
  • Atomic Force Microscopy Imaging of Chromatin in Cancerous and Non-Cancerous Esophageal Cells
Contributors
Date Created
2019-05
Resource Type
  • Text
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